Nortestosterone: endogenous in urine of goats, sheep and mares?†
(Note: The full text of this document is currently only available in the PDF Version )
Saskia Sterk, Henk Herbold, Marco Blokland, Hennie van Rossum, Leendert van Ginkel and Rainer Stephany
Abstract
For a number of species it is known that nortestosterone, either the alpha- or beta-epimer, can be of endogenous origin. For goats and mares similar results have not yet been published. As a follow-up on the experiments with cattle, a large number of urine samples per animal were collected from pregnant goats, sheep and mares. These samples were analysed for the presence of alpha- and beta-nortestosterone and alpha-estradiol using GC-MS. The results show that in the goats and mares studied alpha-nortestosterone is present during pregnancy. In this study no alpha-nortestosterone could be demonstrated in sheep. From our study and recently published data, however, it is proven that alpha-nortestosterone can occur endogenously.
References
- D. Courtot, J. L. Guyot and E. Benoit, Comptes Rendus de L'Académie des Sciences, -III-Sciences de la Vie, 1984 Search PubMed.
- E. Houghton, A. Ginn, P. Teale, M. C. Dumasia and M. S. Moss, Proc. 6th Intern. Conf. Racing Anal. Vet. Hong Kong, 1985, 229 Search PubMed.
- G. Maghuin-Rogister, A. Bosseloire, P. Gaspar, C. Dasnois and G. Pelzer, Ann. Méd. Vet., 1988, 132, 437 Search PubMed.
- L. A. Van Ginkel, R. W. Stephany, P. W. Zoontjes, H. van Rossum, H. van Blitterswijk and J. Zuydendorp, Tijdschrift voor Diergeneeskunde, 1989, 114, 311 Search PubMed.
- G. Debruyckere, C. Van Peteghem, H. F. De Brabander and G. Debackere, The Vet. Quart., 1990, 12, 246 Search PubMed.
- M. Vandenbroeck, G. Van Vyncht, J. De Graeve and G. Maghuin-Rogister, J. Chromatogr., 1991, 564, 405 CrossRef CAS.
- H. D. Meyer, T. Falckenberg, T. Janowski and M. Rapp, Acta Endocrinol., 1992, 126, 369 Search PubMed.
- H. F. De Brabander, J. van Hende, P. Batjoens, L. Hendriks, J. Raus, F. Smets, G. Pottie, L. A. van Ginkel and R. W. Stephany, Analyst, 1994, 119, 2581 RSC.
- A.-S. Clouet, B. Le Bizec, M.-P. Montrade, F. Monteau and F. André, Analyst, 1997, 122, 471 RSC.
- European Commission Decision 93/256/EEC of 14 April 1993, ‘Laying down the methods to be used for detecting residues of substances having a hormonal or thyrostatic action’, Off. J. Eur. Comm., L 118, 1993, 64 Search PubMed.