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DOI: 10.1039/A804932G (Paper) Reference Section for: Analyst, 1998, 123, 2415-2422

Detection of corticosteroids in injection sites and cocktails by MSn†

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Katia De Wasch, Hubert De Brabander, Dirk Courtheyn and Carlos Van Peteghem

Abstract

In the European Union, the use of growth promoting substances such as thyreostats, anabolics (products with estrogenic, androgenic or gestagenic action) and beta-agonists in animal fattening is forbidden. Corticosteroids, such as dexamethasone, although considered catabolic substances, have been administered to food producing animals in order to achieve mass gains. For the analysis of injection sites and of suspect cocktails (found at the farm), a number of HPTLC and HPLC methods are used. However, in injection sites and also in cocktails found at the farm, sometimes many unknown substances are found. In this investigation, a multiple mass spectrometric (MSn) method was developed. The method is based on rapid extraction of the matrix with methanol and direct infusion of the extract into the interface of the mass spectrometer. Tables that summarise the masses of corticosteroids and their possible esters are presented.

References

  1. N. R. Adams and M. R. Sanders, Aust. Vet. J., 1992, 69, 209 Search PubMed.
  2. W. R. Dayton and M. R. Hathaway, in Growth Regulation in Farm Animals—Advances in Meat Research, vol. 7, ed. A. M. Pearson and T. R. DutsonElsevier Applied Science, London, 1991, pp. 17–45 Search PubMed.
  3. L. Istasse, V. de Haan, C. Van Eenaeme, B. Buts, P. Baldwin, M. Gielen, D. Demeyer and J. M. Bienfait, J. Anim. Physiol. Anim. Nutr., 1989, 62, 150 CAS.
  4. P. M. Keen, Vet. Ann., 1987, 27, 45 Search PubMed.
  5. G. Dhondt, R. Ectors, L. Mathys and R. Ducatelle, Vlaams Diergeneesk. Tijdschr., 1993, 62, 35 Search PubMed.
  6. G. De Bruykere and C. Van Peteghem, Anal. Chim. Acta, 1992, 275, 49 CrossRef CAS.
  7. D. Courtheyn, N. VerheyeV. Bakeroot, V. Dal, R. Schilt, H. Hooijerink, E. O. Van Bennekom, W. Haasnoot, P. Stouten and F. A. Huf, in Proceedings of Euroresidue II, Veldhoven, 1993, ed. N. Haagsma, A. Ruiter and P. B. Czedik-Eysenberg, 1993, pp. 251-256 Search PubMed.
  8. K. Vanoosthuyze, L. S. G. Van Poucke, A. C. A. Deloof and C. H. Van Peteghem, Anal. Chim. Acta, 1993, 275, 177 CrossRef CAS.
  9. J. Hoebus, E. Daneels, E. Roets and J. Hoogmartens, J. Planar Chromatogr., 1993, 6, 269 CAS.
  10. D. Courtheyn, J. Vercammen, H. De Brabander, I. Vandereyt, P. Batjoens, K. Vanoosthuyze and C. Van Peteghem, Analyst, 1994, 119, 2557 RSC.
  11. D. G. Watson, J. M. Midgley and C. N. J. McGhee, Rapid Commun. Mass Spectrom., 1989, 1, 8.
  12. J. M. Midgley, D. G. Watson, T. Healy and M. Noble, J. Pharm. Pharmacol., 1987, 39(Suppl.), 51 Search PubMed.
  13. E. Houghton, P. Teale and M. C. Dumasia, Analyst, 1984, 109, 273 RSC.
  14. Y. Kim, T. Kim and W. Lee, Rapid Commun. Mass Spectrom., 1997, 11, 863 CrossRef CAS.
  15. S. Rizea-Savu, L. Silvestro, A. Haag and F. Soergel, J. Mass Spectrom., 1996, 31, 1351 CrossRef.
  16. S. J. Park, Y. J. Kim, H. S. Pyo and J. Park, J. Anal. Toxicol., 1990, 14, 102 CAS.
  17. F. Smets, G. Pottie, H. F. De Brabander, P. Batjoens, L. Hendriks, D. Courtheyn, B. Lancival and Ph. Delahaut, in Analyst, 1994, 119, 2571 Search PubMed.
  18. EEC ( 1993) document 93/256/EEC, Official J. Eur. Commun., 1993, No. L 118/64 Search PubMed.
  19. R. W. Stephany and L. G. Van Ginkel, Fresenius' J. Anal. Chem., 1990, 338, 370 CrossRef CAS.
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