Determination of betamethasone and triamcinolone acetonide by GC-NCI-MS in excreta of treated animals and development of a fast oxidation procedure for derivatisation of corticosteroids†

Abstract

The use of corticosteroids in combination with other hormonal substances has long been known to result in increased mass gain with bovines. Practice has demonstrated, however, that even the single use of a glucocorticoid may result in growth promoting effects. In addition to the popular dexamethasone, more recently other corticosteroids have also been misused for fattening purposes. The first part of this study deals with the detection of two of them, namely betamethasone and triamcinolone acetonide. Betamethasone was administered orally to a cow at a dose of 50 mg d^–1 for 5 d, then later the same cow was injected intramuscularly with a dose of 50 mg of betamethasone dipropionate. Excretion in urine and faeces was followed with both HPLC-enzyme immunoassay and a previously described method based on negative chemical ionization mass spectrometry (NCI-MS) after oxidation. For the triamcinolone acetonide study a cow was treated with 50 mg d^–1 of the drug during a 7 d period. Excretion in faeces was followed with GC-NCI-MS. As triamcinolone acetonide is resistant to the previously described oxidation procedure, however, a hydrolysis step had to be introduced prior to oxidation. In addition to this specific modification necessary for triamcinolone acetonide, in a subsequent part of this study the original oxidation procedure with pyridinium chlorochromate was re-investigated especially to shorten the procedure. With the introduction of potassium dichromate the reaction time could be decreased from 3 h to 10 min.

References

1. D. Courtheyn, J. Vercammen, H. De Brabander, I. Vandenreyt, P. Batjoens, K. Vanoosthuyse and C. Van Peteghem, Analyst, 1994, 119, 2557 and references cited therein.
2. K. De Wasch, H. F. De Brabander, D. Courtheyn and C. Van Peteghem, Analyst, 1998, 123, 2415.
3. M. L. J. Rijckaert, and H. P. J. Vlemmix, The Growth Promoting Effect of Glucocorticosteroids, Department of Chemical Engineering, Eindhoven University of Technology, Eindhoven, 1992.
4. M. J. Groot, P. L. M. Berende, R. Schilt, W. Haasnoot, H. Hooijerink and J. S. Ossenkoppele, De Effecten van Lage Doseringen Betaagonisten al of Niet Gecombineerd met Oestradiol, Methylthiouracil en Dexamethason bij Vleeskalveren, Rikilt-DLO Rapport 94.22, Rikilt-DLO, Wageningen, 1994.
5. L. Istasse, V. De Haan, C. Van Eenaeme, B. Buts, P. Baldwin, M. Gielen, D. Demeyer and J. M. Bienfait, J. Anim. Physiol. Anim. Nutr., 1989, 62, 150.
6. N. R. Adams and M. R. Sanders, Aust. Vet. J., 1992, 69, 209.
7. J. Negriolli, PhD Thesis, Faculty of Sciences and Techniques, University of Nantes, 1997.
8. G. R. Her and J. T. Watson, Biomed. Environ. Mass Spectrom., 1986, 13, 57.
9. M. R. Koupai-Abyazani, N. Yu, B. Esaw and B. Laviolette, J. Anal. Toxicol., 1995, 19, 182.
10. J. March, in Advanced Organic Chemistry: Reactions, Mechanisms, and Structure, ed. D. N. Hume, G. Stork, E. L. King, D. R. Herschbach and J. A. Pople, McGraw-Hill, New York, 1968, p. 344.
11. Ph. Delahaut, P. Jacquemin, Y. Colemonts, M. Dubois, J. De Graeve and H. Deluyker, J. Chromatogr. B, 1997, 696, 203.