Modified determination of dihydrostreptomycin in kidney, muscle and milk by HPLC†

Abstract

A method is presented for the determination of dihydrostreptomycin in milk, muscle and kidney by reversed-phase ion-pair high-performance liquid chromatography and post-column derivatisation with β-naphthoquinone-4-sulfonate prior to fluorescence detection. The new sample work-up procedures include acid precipitation of proteins and, in the case of muscle and kidney, removal of fats by solvent extraction followed by solid phase extraction on a cation exchanger. The fluorescence response was linear from 25 to 2000 µg l^–1 of injected analyte. The detection limits were 10 µg kg^–1 for milk and 15 µg kg^–1 for muscle and kidney and the analyte recoveries were on average 93% for milk, 70% for kidney and 75% for muscle.

References

1. Mlle Par, F. Faure and M. P. Blanquet, Bull. Soc. Pharm. Bordeaux, 1961, 100, 105.
2. F. Faure and M. P. Blanquet, Clin. Acta, Bordeaux, 1964, 9, 292.
3. H. Kubo, Y. Kobayashi and T. Kinoshita, Anal. Chem., 1986, 58, 2653.
4. G. C. Gerhardt, C. D. C. Salisbury and J. D. MacNeil, J. AOAC Int., 1994, 77, 334.
5. V. Hormazabal and M. Yndestad, J. Liq. Chromatogr., Relat. Technol., 1997, 20, 2259.
6. V. Hormazabal and M. Yndestad, J. Liq. Chromatogr., 1995, 18, 2695.