Non-aqueous enzymology approach for improvement of reagentless mediator-based glucose biosensor†

(Note: The full text of this document is currently only available in the PDF Version )

Lilia V. Lukachova, Arkady A. Karyakin, Yulia N. Ivanova, Elena E. Karyakina and S. D. Varfolomeyev


Abstract

The formation of Nafion membranes containing glucose oxidase and dimethylferrocene as a mediator was optimized using a previously reported non-aqueous enzymology approach for biosensor development. Enzyme immobilization in Nafion membranes was carried out from water–organic mixtures with a high content of organic solvent. The mediator based reagentless glucose electrode was tested in a flow injection system. The response towards glucose addition was stable: the reproducibility during 50 assays exceeded 95%. The response was linear over the glucose concentration range 0.5–50 mM.


References

  1. L. C. Clark and C. Lyons, Ann. N.Y. Acad. Sci., 1962, 102, 29 CAS .
  2. A. E. G. Cass, G. Davis, G. D. Francis, H. A. O. Hill, W. G. Aston, I. J. Higgins, E. V. Plotkin, L. D. L. Scott and A. P. F. Turner, Anal. Chem., 1984, 56, 667 CrossRef CAS .
  3. R. F. B. Turner, D. J. Harrison, R. V. Rajotte and H. P. Baltes, Sens. Actuators B, 1990, 1, 561 CrossRef .
  4. C. Y. Chen, E. Tamiya, K. Ishihara, Y. Kosugi, Y. C. Su, N. Nakabayashi and I. Karube, Appl. Biochem. Biotechnol., 1992, 36, 211 Search PubMed .
  5. C. Y. Chen, M. Gotoh, H. Makino, Y. C. Su, E. Tamiya and I. Karube, Anal. Chim. Acta, 1992, 265, 5 CrossRef CAS .
  6. T. J. Ohara, R. Rajagopalan and A. Heller, Anal. Chem., 1994, 66, 2451 CrossRef CAS .
  7. R. M. Wightman, in Biosensor Technology, ed. R. P. Buck, W. E. Hatfield, M. Umana and E. F. Bowden, Marcel Dekker, New York, 1990, p. 39 Search PubMed .
  8. M. G. Garguilo and A. C. Michael, Anal. Chim. Acta, 1995, 307, 291 CrossRef CAS .
  9. E. N. Navera, M. Suzuki, E. Tamiya, T. Takeuchi and I. Karube, Electroanalysis, 1993, 5, 17 CAS .
  10. W. Matuszewski, M. Trojanowicz and A. Lewenstam, Electroanalysis, 1990, 2, 607 CAS .
  11. Y. Zhang, Y. Hu, G. S. Wilson, D. Moatti-Sirat, V. Poitout and G. Reach, Anal. Chem., 1994, 66, 1183 CrossRef CAS .
  12. I. Rubinstein and A. J. Bard, J. Am. Chem. Soc., 1980, 102, 6641 CrossRef .
  13. I. Rubinstein and A. J. Bard, J. Am. Chem. Soc., 1981, 103, 5007 CrossRef CAS .
  14. S. Dong, B. Wang and B. Liu, Biosens. Bioelectron., 1991, 7, 215 CrossRef CAS .
  15. R. S. Brown and J. H. Luong, Anal. Chim. Acta, 1995, 310, 419 CrossRef CAS .
  16. J. K. Harkness, O. J. Murphy and G. D. Hitchens, J. Electroanal. Chem., 1993, 357, 261 CrossRef CAS .
  17. H. Liu and J. Deng, Electrochim. Acta, 1995, 40, 1845 CrossRef CAS .
  18. A. A. Karyakin, E. E. Karyakina, L. Gorton, O. A. Bobrova, L. V. Lukachova, A. K. Gladilin and A. V. Levashov, Anal. Chem., 1996, 68, 4335 CrossRef CAS .
  19. R. Appelqvist, G. Marko-Varga, L. Gorton, A. Torstensson and G. Johansson, Anal. Chim. Acta, 1985, 169, 237 CrossRef CAS .
Click here to see how this site uses Cookies. View our privacy policy here.