Philip Britz-Mckibbin, Andrea R. Kranack, Alison Paprica and David D. Y. Chen
A simple and robust method for the separation and quantification of epinephrine in dental anesthetic solutions was developed. The method allows the direct injection of high salt solutions without sample pre-treatment. Large sample plugs (5.7% of the total capillary length) are used for epinephrine determination by selective analyte focusing in capillary electrophoresis. The concentration detection limit for epinephrine is about 5.0 × 10–7 M (90 ng ml–1) with a commercial UV detector. The separation protocol was validated in terms of its precision, linearity, accuracy and specificity.