Time resolved fluorimmunoassay of abscisic acid in potato leaves

Abstract

A monoclonal antibody to abscisic acid (ABA) was obtained and employed in a time-resolved fluoroimmunoassay for ABA content in potato leaf extracts. The useful range of the logarithmic calibration graph was 0.05–50 pmol well^–1. Comparison with ELISA gave the linear regression y = 0.99x + 1.93; the coefficient of correlation was r = 0.96.

References

1. E. W. Weiler, H. Schnabl and C. Hornberg, Planta, 1982, 154, 24.
2. C. F. Morris, R. J. Anderberg, P. J. Goldmark and M. K. Walker-Simmons, Plant Physiol., 1991, 95, 814.
3. P. H. Rosher, H. G. Jones and P. Hedden, Planta, 1984, 165, 91.
4. E. W. Weiler, Physiol. Plant, 1982, 54, 510.
5. M. J. Harris, W. H. Outlaw Jr., R. Mertens and E. W. Weiler, Proc. Natl. Acad. Sci. U.S.A., 1988, 85, 2584.
6. E. W. Weiler, Planta, 1980, 148, 262.
7. S. A. Quarrie and G. Galfre, Anal. Biochem., 1985, 151, 389.
8. M. Walker-Simons, M. J. T. Reaney, S. A. Quarrie, P. Perata, P. Vernieri and S. Abrams, Plant Physiol., 1991, 95, 46.
9. R. A. Evangelista, A. Pollak, B. Allore, E. F. Templeton, R. C. Morton and E. P. Diamandis, Clin. Biochem., 1988, 21, 173.
10. M. A. Bacigalupo, A. Ius and G. Meroni, Analyst, 1994, 119, 2813.
11. J. Kropf, E. Quitte and A. M. Gressnar, Anal. Biochem., 1991, 197, 258.
12. A. Ius, L. Ferrara, G. Meroni and M. A. Bacigalupo, Fresenius' J. Anal. Chem., 1990, 336, 235.
13. S. Cammisuli and L. Wofry, J. Immunol., 1976, 117, 1695.
14. S. Fazekas de Saint Groth and D. Sheidegger, J. Immunol. Methods, 1980, 35, 1.