Cyanide determination in biological fluids using a microdiffusion method with a flow system and polarographic detection

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Paulo C. do Nascimento and Denise Bohrer


Abstract

The report describes a method for the automated polarographic determination of cyanide as tetracyanonickelate(II) anion complex in a gas-diffusion flow system. The volatile cyanide, existing in whole blood, plasma and urine samples, was measured after gas-diffusion using 8 × 10–5 mol l–1 hexaaminenickel solution as acceptor. The linear range of calibration, for measurements at the hanging mercury-drop electrode (HMDE), was from 0.1 to 2.0 µg cyanide with r = 0.998. The RSD was, respectively, 3.4 and 1.2% (n = 5) for 0.4 µg cyanide measured with and without the flow-system configuration. Detection limits of 7.4 µg l–1 were calculated using the flow system and the method was compared with the classical method using Cavet flasks. Parameters that affect the cyanide determination in the proposed method, such as acceptor solution, pH, flow rate and temperature, were investigated.


References

  1. M. B. Jacobs, in The Analytical Toxicology of Industrial Inorganic Poisons, Interscience, New York, 1967, vol. 22, p. 721 Search PubMed.
  2. M. S. Upadhyay and V. K. Gupta, Analyst, 1984, 109, 1619 RSC.
  3. J. Epstein, Anal. Chem., 1947, 19, 272 CrossRef CAS.
  4. R. L. Moran, G. E. Isom and J. L. Way, Toxicol. Appl. Pharmacol., 1979, 50, 323 CrossRef.
  5. S. Baar, Analyst, 1966, 91, 268 RSC.
  6. J. J. Rosentreter and R. K. Skogerboe, Anal. Chem., 1991, 63, 682 CrossRef CAS.
  7. P. McCarthy and R. W. Klusman, Anal. Chem., 1993, 65, 244R CAS.
  8. P. McCarthy, R. W. Klusman and S. W. Cowling, Anal. Chem., 1991, 63, 301R.
  9. P. McCarthy, R. W. Klusman and J. A. Rice, Anal. Chem., 1989, 61, 269R.
  10. C. Pohlandt and M. J. Hemmings, S. Afr. J. Chem., 1988, 41, 136 Search PubMed.
  11. C. Pohlandt, S. Afr. J. Chem., 1984, 37, 133 Search PubMed.
  12. P. Sillinger, Plating Surf. Finishing, 1985, 72, 82 Search PubMed.
  13. M. W. Scoggings, Anal. Chem., 1970, 42, 301 CrossRef.
  14. M. W. Scoggings, Anal. Chem., 1972, 44, 1294 CrossRef.
  15. G. Schwedt and P. C. do Nascimento, Anal. Chim. Acta., 1993, 282, 755.
  16. P. C. do Nascimento, D. Bohrer and J. Tramontina, Analyst, 1997, 122, 835 RSC.
  17. E. E. Archer, Analyst, 1958, 83, 571 RSC.
  18. H. B. Mark, Jr. and C. N. Reilley, Anal. Chem., 1963, 35, 195 CrossRef.
  19. H. B. Mark, Jr. and C. N. Reilley, J. Electroanal. Chem., 1962, 4, 189 CrossRef.
  20. H. B. Mark, Jr., Anal. Chem., 1964, 36, 940 CrossRef.
  21. H. B. Mark, Jr., J. Electroanal. Chem., 1964, 7, 276 CrossRef.
  22. J. M. L. Fonseca and D. Fojón, Anal. Chim. Acta., 1990, 230, 97 CrossRef.
  23. C. Giovedi and V. Tokoro, J. Braz. Chem. Soc., 1997, 8, 187 Search PubMed.
  24. A. S. Perez and V. De Frutos, Anal. Chim. Acta., 1995, 317, 319 CrossRef.
  25. J. A. Nóbrega, M. D. Capelato and R. Gama, J. Braz. Chem. Soc., 1994, 5, 91 Search PubMed.
  26. C. J. Vesey and J. Wilson, J. Pharm. Pharmacol., 1978, 30, 20 Search PubMed.
  27. H. J. Gibitz and M. Geldmacher, Klinisch-toxikologische Analytik bei akuten Vergiftungen und Drogenmissbrauch, VCH, New York, 1989, p. 196 Search PubMed.
  28. J. C. Valentour, V. Aggarwal and I. Sunshine, Anal. Chem., 1974, 46, 924 CrossRef CAS.
  29. R. W. Darr, T. L. Capson and F. D. Hileman, Anal. Chem., 1980, 52, 1379 CrossRef CAS.
  30. F. McAuley and D. S. Reive, J. Anal. Toxicol., 1983, 7, 214.
  31. J. Zamecnick and J. Tam, J. Anal. Toxicol., 1987, 11, 47.
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