Effect of non-ionic surfactants as mobile phase additives on the fluorescence intensity of dansyl derivatives of biogenic amines in high-performance thin-layer chromatography

Abstract

The effects of non-ionic surfactants as mobile phase additives on the fluorescence intensity of dansyl derivatives of biogenic amines in high-performance thin-layer chromatography (HPTLC) were investigated. The dansyl derivatives of putrescine, cadaverine, spermidine and spermine were separated by HPTLC with silica gel as stationary phase and an eluent consisting of chloroform–triethylamine (2 + 1 v/v) containing 5% m/v of polyoxyethylene-10-lauryl ether (POLE). A fibre-optic-based fluorescence instrument for in situ scanning was used for quantitative measurements. The compounds were determined over the range 0.5–85 ng, with relative standard deviations between 0.44 and 1.16% and detection limits in the range 0.28–0.39 ng. It is shown that the presence of POLE in the mobile phase enhances both the fluorescence intensity and the signal-to-noise ratio of the chromatographed dansyl derivatives. The method was applied to the determination of biogenic amines in plant tissue and beer samples.

References

1. A. W. Galston and R. Kaur-Sawhney, Plant Physiol., 1990, 94, 406.
2. P. Evans and R. Malmberg, Annu. Rev. Plant Physiol. Plant Mol. Biol., 1989, 40, 235.
3. P. Malle, M. Valle and S. Bouquelet, J. Assoc. Off. Anal. Chem., 1996, 79, 43.
4. E. T. Ryser, S. L. Taylor and E. H. Marth, System Appl. Microbiol., 1984, 5, 545.
5. S. L. Taylor, CRC Crit. Rev. Toxicol., 1986, 17, 91.
6. J. L. Mietz and E. Karmas, J. Food Sci., 1977, 42, 155.
7. N. Seiler, J. Chromatogr., 1971, 63, 97.
8. K. Khayria, A. M. Ayesh and A. R. Shalaby, J. Agric. Food Chem., 1995, 43, 134.
9. K. D. Henry Chin and P. E. Koehler, J. Food Sci., 1983, 48, 1826.
10. S. Yamamoto, H. Itano, H. Kataoka and M. Makita, J. Agric. Food Chem., 1982, 30, 435.
11. W. F. Staruszkiewicz and J. F. Boun, J. Assoc. Off. Anal. Chem., 1981, 64, 584.
12. M. Bonilla, L. G. Enriquez and H. M. McNair, J. Chromatogr. Sci., 1997, 35, 53.
13. C. Buteau, C. L. Duitschaever and G. C. Ashton, J. Chromatogr., 1984, 284, 81.
14. H. M. L. J. Joosten and C. Olieman, J. Chromatogr., 1986, 356, 311.
15. G. Chiavari, G. C. Galletti and P. Vitali, J. Chromatogr., 1989, 27, 216.
16. S. Suzuki, K. Kobayashi, J. Noda, T. Suzuki and K. Takama, J. Chromatogr., 1990, 508, 225.
17. H. M. H. van Eijk, D. R. Rooyakkers and N. E. P. Deutz, J. Chromatogr., 1996, 730, 115.
18. D. W. Armstrong and R. Q. Terrill, Anal. Chem., 1979, 51, 2160.
19. W. L. Hinze, D. Y. Pharr, Z. S. Fu and W. G. Burker, Anal. Chem., 1989, 61, 422.
20. L. Lepri, V. Coas and P. G. Desideri, J. Planar Chromatogr., 1990, 3, 533.
21. L. Lepri, V. Coas and P. G. Desideri, J. Planar Chromatogr., 1991, 4, 338.
22. S. N. Shtykov, E. G. Sumina, E. V. Parshina and S. S. Lopukhova, J. Anal. Chem., 1995, 50, 747.
23. W. R. G. Baeyens and B. Lin Ling, J. Planar Chromatogr., 1988, 1, 198.
24. F. De Croo, G. A. Bens and P. De Moerloose, J. High Resolut. Chromatogr. Chromatogr. Commun., 1980, 3, 423.
25. B. Lin Ling, W. R. G. Baeyens, H. Marysael and K. Stragierm, J. High Resolut. Chromatogr., 1989, 12, 345.
26. A. Navas Díaz, Anal. Chim. Acta, 1991, 255, 297.
27. W. Funk, V. Dammann, T. Couturier, J. Schiller and L. Völker, J. High Resolut. Chromatogr. Chromatogr. Commun., 1986, 9, 224.
28. H. E. Flores and A. W. Galston, Plant Physiol., 1982, 69, 701.
29. R. M. Linares, J. H. Ayala, A. M. Afonso and V. Gonzalez Díaz, Anal. Lett., 1998, 31(3) in press.
30. Y. Liang, M. E. Baker, D. A. Gilmore and M. B. Denton, J. Planar Chromatogr., 1996, 9, 247.
31. D. Hornero and A. Garrido, Analyst, 1994, 119, 2037.
32. H. Ohta, Y. Takeda, K. Yoza and Y. Nogata, J. Chromatogr., 1993, 628, 199.
33. T. Murashige and F. Skoog, Physiol. Plant., 1962, 15, 473.
34. M. L. Izquierdo-Pulido, M. C. Vidal-Carou and A. Marine-Font, J. AOAC Int., 1993, 76, 1033.