Enzyme-linked immunosorbent assay for ondansetron captured from airborne samples

Abstract

A simple and relatively rapid enzyme-linked immunosorbant assay method for the anti-emetic drug ondansetron has been developed for its quantitation in solution. This has been optimised for use with samples that have been obtained following extraction of filters after the drug’s capture from air samples in the workplace. The assay has the sample throughput (40 duplicate samples in 3 h), specificity, sensitivity (LOD of 10.5 ng drug ml^–1) and precision (RSD < 11%) necessary for its use in determining airborne concentrations of ondansetron in such samples as part of an occupational health and hygiene monitoring programme.

References

1. Substance Information Sheet for Ondansetron, GlaxoWellcome Operations, Ware, Herts., UK.
2. P. V. Colthup, C. C. Felgate, J. L. Palmer and N. L. Scully, J. Pharm. Sci., 1991, 80, 868.
3. J. Liu and J. T. Stewart, Chromatogr. B, Biomed. Appl., 1997, 694, 179.
4. M. Siluveru and J. T. Stewart, J. Chromatogr. B, Biomed. Appl., 1997, 691, 217.
5. P. Sanwald, M. David and J. Dow, J. Chromatogr. B, Biomed. Appl., 1996, 678, 53.
6. J. M. Van Emon and C. L. Gerlach, in Environmental Immunochemical Methods, Symposium Series 646, ed. Van Emon, J. M., Gerlach, C. L., and Johnson, J. C., American Chemical Society, Washington, DC, 1996, pp. 2–8.
7. R. Cumming, C. Farrell, I. Nitescu, F. J. Rowell and L. X. Tang, Anal. Chim. Acta, 1995, 311, 377.
8. F. J. Rowell, R. H. Cumming and I. Nitescu, Anal. Chim. Acta, 1995, 316, 247.
9. S. A. Wring, R. M. Rooney, C. P. Goddard, I. Waterhouse and W. N. Jenner, Pharm. Biomed. Anal., 1994, 12, 361.
10. B. Eistert, G. Kilpper and J. Goring, Chem. Ber, 1969, 102, 1379.
11. G. R. Clemo and D. G. I. Felton, J. Chem. Soc., 1951, 700.
12. G. V. Bhide, N. L. Tikotkar and B. D. Tilak, Chem., Ind., 1957, 363.
13. J. Ballantine, C. Barret and R. Beer, J. Chem. Soc., 1957, 2227.
14. F. J. Rowell, Z.-F. Mioa, R. N. Reeve and R. H. Cumming, Analyst, 1997, 122, 1505.