Determination of tylosin residues in pig tissues using high-performance liquid chromatography

Abstract

In accordance with the maximum residue limit of 100 µg kg^–1 established by EU legislation, a simple and sensitive high-performance liquid chromatographic (HPLC) method was developed for the measurement of tylosin residues in pig tissues (fat, kidney, liver and muscle). Tylosin, a macrolide antibiotic, is extracted with water–methanol and cleaned-up by solid-phase extraction (SPE) on cation-exchange cartridges using methanol elution. Tylosin was determined by reversed-phase HPLC with UV detection at 280 nm and the mean recovery from pig tissues fortified in the range 50–200 µg kg^–1 was 70–85%, with intra- and inter-day RSDs in the ranges 3.4–9.1 and 3.9–10.1% respectively.

References

1. Commission Regulation 92/675/EEC, March 1992, 18, Off. J. Eur. Commun., 1992, L.73, 8.
2. B. Delepine, D. Hurtaud and P. Sanders, Analyst, 1994, 119, 2717.
3. W. A. Moats, E. W. Harris and N. C. Steele, J. Assoc. Off. Anal. Chem., 1985, 68, 413.
4. M. G. Lauridsen, C. Lund and M. Jacobsen, J. Assoc. Off. Anal. Chem., 1988, 71, 921.
5. B. Delépine, D. Hurtaud-Pessel and P. Sanders, J. Assoc. Off. Anal. Chem., 1996, 79, 397.
6. FAO/WHO, Monographs Prepared by the Thirty-eighth Meeting of the Joint Expert Committee on Food Additives, Rome, 22–31 January 1991.
7. Commission Decision 93/256/EEC, April 1993, 14, Off. J. Eur. Commun., 1993, L.118, 64.
8. Commission Regulation 97/1838/EEC, September 1997, 24, Off. J. Eur. Commun., L.263, 14.