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DOI: 10.1039/A706114E (Paper) Reference Section for: Analyst, 1998, 123, 287-290

Spectrofluorimetric determination of vitamin K3

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J. J. Berzas Nevado and M. A. Gómez Laguna

Abstract

A spectrofluorimetric method for determining vitamin K3 (menadione) is described. Because this compound is not naturally fluorescent, a prior reduction of vitamin K3 was effected by using SnCl2 stabilized in glycerol to obtain the reduced fluorescent product (naphthohydroquinone). The fluorescence response (λex = 334 nm; λem = 427 nm) was linear up to 180 µg l–1 of vitamin K3 with a detection limit of 0.62 µg l–1 and a determination limit of 2.06 µg l–1. The method was applied to commercial preparations with good results. The recoveries achieved in all cases varied between 104.4 and 98.92%. Vitamins such as folic acid, pyridoxamine and ascorbic acid did not interfere.

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