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DOI: 10.1039/A705558G (Paper) Reference Section for: Analyst, 1998, 123, 99-103

Immunological effects of occupational exposure to metallic mercury in the population of T-cells and NK-cells†

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Paulin Moszczyński

Abstract

This paper presents a study of the counts of lymphocytes, (CD3+)T-cells, (CD4+)T-helper and (CD8+)T-suppressor and (CD16+)NK-cells in the peripheral blood of 101 males with a history of occupational exposure to metallic mercury vapours (Hg0) and in 36 males without this exposure. These workers were divided depending on the duration of exposure: 37 males with a short-term history of exposure to Hg0 (up to 10 years) and 64 males with a history of long-term exposure (10 to 37 years). For the determination of T-cell populations monoclonal antibodies were used in indirect immunofluorescence tests. The time weighted average of mercury concentrations in air was 0.028 mg m–3. Mercury concentration in the urine of the exposed subjects ranged from 20–260 µg dm–3, and in blood it was from 4 to 72 µg dm–3. Stimulation of the T-cell line was noted as evidenced by increased numbers of (CD3+)T-cells, (CD4+)T-helper and (CD8+)T-suppressor cells in the workers with <10 or >10 years’ exposure to Hg0. Lower increase count of (CD3+)T-cells and (CD4+)T-helper cells than (CD8+)T-suppressor cells was the cause of decreased values in the (CD3+)T/(CD8+)T-suppressor ratio and (CD4+)T-helper/(CD8+)T-suppressor ratio in the workers with <10 or >10 years’ of exposure. Moreover, no changes were observed in the T-cell populations between workers with <10 and those with >10 years’ exposure. In addition, statistical analysis of the effects of age and duration of exposure to Hg0 on the studied immunological parameters indicates that exposure duration may affect some of the values. These quantitative changes of T-cell population as well as changes of the (CD3+)T/(CD8+)T-suppressor and (CD4+)T-helper/ (CD8+)T-suppressor ratio have been proposed as immunological indicators of exposure to Hg0, which can be used for monitoring and to explain the origin of autoimmunity disorders induced by metallic mercury.

References

  1. D. A. Lawrence, in Immunotoxicology and Immunopharmacology, ed. Munson, A. R., and Amos H., Raven Press, New York, 1985, pp. 341–353 Search PubMed.
  2. J. T. Zelikoff, R. Smialowicz, P. E. Bigazzi, R. A. Goyer, D. A. Lawrence, H. I. Maibach and D. Gardner, Fundam. Appl. Toxicol., 1994, 22, 1 CrossRef CAS.
  3. K. Hirokawa and Y. Hayashi, Acta Pathol. Jpn., 1980, 30, 23 Search PubMed.
  4. N. G. Ilback, Toxicology, 1991, 67, 117 CrossRef CAS.
  5. S. Nakatsuru, J. Oohasshi, H. Nozaki, S. Nakada and N. Imura, Toxicology, 1985, 36, 297 CrossRef CAS.
  6. K. Nordlind, Int. Archs. Allergy Appl. Immun., 1983, 72, 177 Search PubMed.
  7. V. L. M. Esnault, P. W. Mathieson, S. Thiru, D. B. G. Oliveira and C. Martin-Lockwood, Lab. Invest., 1992, 67, 114 Search PubMed.
  8. P. Hultman and S. Enestrom, Toxicol. Appl. Pharmacol., 1992, 113, 199 CrossRef CAS.
  9. C. D. Pusey, C. Bowman, A. Morgan, A. P. Weetman, B. Hartley and C. M. Lockwood, Clin. Exp. Immunol., 1990, 81, 76 CAS.
  10. Y. Jiang and G. Moller, Int. Immunol., 1996, 8, 1729 CAS.
  11. J. Fillion, R. Baccala, C. Pannetier, J. Kuhn, P. Druet and B. Bellon, Int. Immunol., 1997, 9, 263 CrossRef CAS.
  12. M. Holst and K. Nordlind, Int. Arch. Allergy Appl. Immun., 1988, 87, 381 Search PubMed.
  13. R. C. R. Perlingeiro and M. L. S. Queiroz, Int. J. Immunopharmacol., 1994, 16, 1011 CrossRef CAS.
  14. B. J. Shenker, C. Rooney, L. Vitale and I. M. Shapiro, Immunopharmacol. Immunotoxicol., 1992, 14, 539 CAS.
  15. K. Izdebska-Szymona and J. Kopeć-Szlezak, Pol. J. Immunol., 1994, 19, 179 Search PubMed.
  16. P. Moszczyński, P. Moszczyński, jr., S. Słowiński, S. Bem and R. Bartus, J. Hyg. Epidemiol. Microbiol. Immunol., 1991, 35, 351 Search PubMed.
  17. P. Moszczyński, J. Rutowski, S. Słowiński, S. Bem and D. Jakus-Stoga, Arch. Med. Res., 1996, 27, 503 CAS.
  18. D. E. Bigazzi, in Immunotoxicology and Immunopharmacology, ed. Munson, A. R., and Amos, H., Raven Press, New York, 1985, pp. 277–290 Search PubMed.
  19. J. H. Michaelson, J. P. McCoy, P. Hirszel and P. E. Bigazzi, Surv. Synth. Path. Res., 1985, 4, 401 Search PubMed.
  20. M. L. S. Queiroz, R. C. R. Perlingeiro, D. C. M. Dantas, J. M. A. Bizzacchi and E. M. De Capitani, Pharmacol. Toxicol., 1994, 74, 72 Search PubMed.
  21. V. Bencko, V. Wagner, M. Wagnerova and V. Ondrejcak, J. Hyg. Epidemiol. Microbiol. Immunol., 1990, 34, 9 Search PubMed.
  22. S. Langworth, C. G. Elinder and K. S. Sundqvist, Scand. J. Work Environ. Health, 1993, 19, 405 Search PubMed.
  23. J. P. Gromiec and T. Rogaczewska, Med. Pr., 1989, 5, 300 Search PubMed.
  24. J. K. Piotrowski, B. Trojanowska and E. M. Mogilnicka, Int. Arch. Occup. Environ. Health, 1975, 35, 245 CAS.
  25. G. Wallis and T. Barber, J. Occup. Med., 1982, 7, 590 Search PubMed.
  26. J. Pilch, J. Łaskawiec and J. Lisiewicz, Folia Heamatol., 1988, 115, 727 Search PubMed.
  27. X. Tan, C. Tang, A. F. Castoldi, L. Manzo and L. G. Costa, J. Toxicol. Environ. Health, 1993, 38, 159 CAS.
  28. M. Kubicka-Muranyi, J. Kremer, N. Rottmann, B. Lubben, R. Albers, N. Bloksma, R. Luhrmann and E. Gleichmann, Int. Arch. Allergy Immunol., 1996, 109, 11 Search PubMed.
  29. P. Hultman, U. Johansson and F. Dagnaes-Hansen, J. Autoimmun., 1995, 8, 809 CAS.
  30. R. R. White and R. L. Brandt, J. Am. Dent. Assoc., 1976, 92, 1204 Search PubMed.
  31. D. J. Eedy, D. Burrows, T. Clifford and A. Fay, J. Prosthet. Dent., 1990, 63, 593 CAS.
  32. D. W. Eggleston, J. Prosthet. Dent., 1984, 51, 617 CAS.
  33. M. Wilhelm, P. Dunninger, R. Ruppel, H. P. Tony, K. Wilms and B. Klaiber, Clin. Invest., 1992, 70, 728 Search PubMed.
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