Effects of copper and zinc ions on the germicidal properties of two popular pharmaceutical antiseptic agents cetylpyridinium chloride and povidone-iodine†

Abstract

The effects of copper and zinc ions on the rate of killing of Gram-negative bacterium Pseudomonas aeruginosa, Gram-positive bacterium Staphylococcus aureus and fungal yeast Candida albicans by antiseptic agents cetylpyridinium chloride and povidone-iodine (Betadine) were investigated. In the 48 test cases copper and zinc ions clearly potentiated the antiseptic agents in 28 (58.3%) cases and exhibited an improved (not clear potentiation) activity in 15 (31.3%) cases. In five (10.4%) cases there was no change in the antiseptics’ antimicrobial activity. In general zinc potentiated the antiseptic agents more than copper. If an ‘improved activity’ was the only criterion for this study, then a more rapid antimicrobial effect was observed in 43 out of the 48 test cases, i.e., 90%.

References

1. D. Wedderbum, J. Soc. Cosmet. Chem., 1958, 9, 210.
2. S. R. Kohn, L. Gershenfeld and M. Barr, J. Pharm. Sci., 1963a, 52, 967.
3. S. R. Kohn, L. Gershenfeld and M. Barr, J. Pharm. Sci., 1963b, 52, 1126.
4. M. R. W. Brown and E. R. Garrett, J. Pharm. Sci., 1964, 53, 179.
5. M. R. W. Brown and R. M. E. Richards, J. Pharm. Pharmacol., 1964, 16(Supplement), 51T.
6. A. Albert, Nature (London), 1953, 458, 201.
7. A. Albert, in Selective toxicity. The physicochemical basis of therapy, Chapman and Hall, London, 5th edn., 1973, pp. 336–337.
8. A. Albert, C. W. Rees and A. J. H. Tomlinson, Br. J. Exp. Pathol., 1956, 37, 500.
9. R. A. MacLeod and E. E. Snell, J. Bacteriol., 1950, 59, 783.
10. I. S. Ross, Trans. Br. Mycol. Soc., 1975, 64(2), 175.
11. Matindale—The Extra Pharmacopoeia, ed. Reynolds, J. E. F., and Prasad, A. B., The Pharmaceutical Press, London, 28th edn., 1982, pp. 930–931.
12. J. E. Loveless and H. A. Painter, J. Gen. Microbiol., 1968, 52, 14.
13. B. L. Vallee and D. D. Ulmer, Ann. Rev. Biochem., 1972, 41, 91.
14. H. Sigel, in Concepts on metal toxicity. Metal ions in biological systems. Marcel Dekker, New York, 1st edn., vol. 20, 1986, p. 41, 51.
15. P. Wanda, J. Cupp, W. Snipes, A. Keith, T. Rucinsky, L. Polish and J. Sands, Antimicrob. Agents Chemother., 1976, 10(1), 96.
16. S. Ohnishi and T. Ito, Biochemistry, 1974, 13(5), 881.
17. E. D. Weinberg, Further studies on metallic ion reversal of oxytetracycline: Reversal of drug-inactivated cells by Mg⁺⁺. Antibiotics Annual, Medical Encyclopedia Inc., New York, 1955(as per Weinberg in 1957), pp. 169–173.
18. E. D. Weinberg, Bacteriol. Rev., 1957, 21, 46.
19. R. G. Eagon and M. A. Asbell, J. Bacteriol., 1969, 97(2), 812.