Determination of codeine, 6-methoxycodeine and thebaine using capillary electrophoresis with tris(2,2′-bipyridyl)ruthenium(II) chemiluminescence detection

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Neil W. Barnett, Benjamin J. Hindson, Simon W. Lewis and Stuart D. Purcell


Abstract

A simple and robust capillary electrophoresis chemiluminescence detection system for the determination of codeine, 6-methoxycodeine and thebaine is described, based upon the reaction of these analytes with chemically generated tris(2,2′-bipyridyl)ruthenium(III) prepared in sulfuric acid (0.05 M). The reagent solution was contained in a glass detection cell which also held both the capillary and the cathode. The resultant chemiluminescence was monitored directly using a photomultiplier tube mounted flush against the base of the detection cell. The methodology, which incorporated a field amplification sample introduction procedure, realised detection limits (3σ baseline noise) of 5 × 10–8M for both codeine and 6-methoxycodeine and 1 × 10–7M for thebaine. The relative standard deviations of the migration times and the peak areas for the three analytes ranged from 2.2 up to 2.5% and 1.9 up to 4.6%, respectively.


References

  1. T. Hara, S. Okamura, S. Kato, J. Yokogi and R. Nakajima, Anal. Sci., 1991, 7, 261 CAS.
  2. T. Hara, J. Yokogi, S. Okamura, S. Kato and R. Nakajima, J. Chromatogr., 1993, 652, 361 CrossRef CAS.
  3. N. Wu and C. W. Huie, J. Chromatogr., 1993, 634, 309 CrossRef CAS.
  4. T. Hara, H. Nishida and R. Nakajima, Anal. Sci., 1994, 10, 823 CAS.
  5. T. Hara, S. Kayama, H. Nishida and R. Nakajima, Anal. Sci., 1994, 10, 223 CAS.
  6. T. Hara, H. Nishida, S. Kayama and R. Nakajima, Bull. Chem. Soc. Jpn., 1994, 67, 1193 CAS.
  7. T. D. Staller and M. J. Sepaniak, Instrum. Sci. Technol., 1995, 23, 235 CAS.
  8. K. Tsukagoshi, A. Tanaka, R. Nakajima and T. Hara, Anal. Sci., 1996, 12, 525 CAS.
  9. K. Tsukagoshi, Y. Okumura, H. Akasaka, R. Nakajima and T. Hara, Anal. Sci., 1996, 12, 869 CAS.
  10. K. Tsukagoshi, H. Akasaka, R. Nakajima and T. Hara, Chem. Lett., 1996, 6, 467.
  11. K. Tsukagoshi, S. Fujimura and R. Nakajima, Anal. Sci., 1997, 13, 279 CAS.
  12. M. F. Regehr and F. E. Regnier, J. Capillary Electrophor., 1996, 3, 117 Search PubMed.
  13. B. Huang, J.-J. Li and J.-K. Cheng, Gaodeng Xuexiao Huaxue Xuebao, 1996, 17, 529 Search PubMed.
  14. S.-Y. Liao and C.-W. Whang, J. Chromatogr. A, 1996, 736, 247 CrossRef CAS.
  15. B. Huang, L. Zhang, J.-J. Li and J.-K. Cheng, Anal. Chem., 1996, 68, 2366 CrossRef CAS.
  16. B. Huang, J.-J. Li and J.-K. Cheng, Sepu, 1995, 13, 430 Search PubMed.
  17. S.-Y. Liao, Y.-C. Chao and C.-W. Whang, J. High Resolut. Chromatogr., 1995, 18, 667 CAS.
  18. S. D. Gilman, C. E. Silverman and A. G. Ewing, J. Microcolumn Sep., 1994, 6, 97 CAS.
  19. R. Dadoo, A. G. Seto, L. A. Colon and R. N. Zare, Anal. Chem., 1994, 66, 303 CrossRef CAS.
  20. J. Y. Zhao, J. Labbe and N. J. Dovichi, J. Microcolumn Sep., 1993, 5, 331 CAS.
  21. R. Dadoo, L. A. Colon and R. N. Zare, J. High Resolut. Chromatogr., 1992, 15, 133 CAS.
  22. M. A. Ruberto and M. L. Grayeski, J. Microcolumn Sep., 1994, 6, 545 CAS.
  23. M. A. Ruberto and M. L. Grayeski, Anal. Chem., 1992, 64, 2758 CrossRef CAS.
  24. Y. Lee and C. Whang, J. Chromatogr. A, 1997, 771, 379 CrossRef CAS.
  25. G. A. Forbes, T. A. Nieman and J. V. Sweedler, Anal. Chim. Acta, 1997, 347, 289 CrossRef CAS.
  26. K. Tsukagoshi, K. Miyamoto, E. Saiko, R. Nakajima, T. Hara and K. Fujinaga, Anal. Sci., 1997, 13, 639 CrossRef CAS.
  27. J. A. Dickson, M. M. Ferris and R. E. Milofsky, J. High Resolut. Chromatogr., 1997, 20, 643 CAS.
  28. M. Korman, J. Vindevogel and P. Sandra, J. Microcolumn Sep., 1993, 5, 525 CAS.
  29. R. B. Taylor, A. S. Low and R. G. Reid, J. Chromatogr., B: Biomed. Appl., 1996, 675, 213 CrossRef CAS.
  30. N. W. Barnett, T. A. Bowser, R. D. Gerardi and B. Smith, Anal. Chim. Acta, 1996, 318, 309 CrossRef CAS.
  31. J. A. Broomhead, C. G. Young and P. Hood, Inorg. Syn., 1990, 28, 338 CAS.
  32. R. D. Gerardi, N. W. Barnett and P. Jones, Anal Chim. Acta, 1998, submitted for publication Search PubMed.
  33. D. S. Burgi and R. L. Chien, Anal. Chem., 1991, 63, 2042 CrossRef CAS.
  34. R. D. Gerardi, N. W. Barnett and S. W. Lewis, Anal. Chim. Acta, 1998, in press Search PubMed.
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