Detection of nitric oxide and nitrite by using a Rhodamine-type fluorescent indicator

Abstract

A unique, colorless Rhodamine derivative, Rhodamine B hydrazide (RBH), was shown to react with nitrite in acidic pH to produce an absorption at 561 nm. The sensitivity of RBH toward nitrite was higher than conventional methods. RBH also demonstrated to give a fluorescence at 581 nm when incubated with an NO donor at neutral pH, and the reaction was enhanced by using an NO scavenger, Carboxy-PTIO.

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