Sensitive chromogenic substrate for detecting peroxidase activity

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Makoto Mizoguchi and Munetaka Ishiyama


Abstract

A novel compound, N,N′-bis(2-hydroxy-3-sulfopropyl)- tolidine, disodium salt, was synthesized as a substrate for peroxidase, and its applicability for use in enzyme immunoassays was examined in DNA damage detection assays.


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