Supercritical fluid extraction and supercritical fluid chromatography of vitamin E in pharmaceutical prepartions

(Note: The full text of this document is currently only available in the PDF Version )

A. Salvador, Ma A. Jaime, M. de la Guardia and G. Becerra


Abstract

Vitamin E contained in solid pharmaceutical and dietetic products was extracted from samples by supercritical fluid extraction and determined by supercritical fluid chromatography. Experimental studies on factors affecting extraction quantitatively were conducted in order to select the most appropriate conditions. Samples were weighed (0.5–1 g) in polymer cartridges and were extracted by CO2 using a constant flow (2 ml min1) during 10–30 min at 400 atm and 60 °C. The extracts were collected into glass vials containing dichloromethane and subsequently transferred to calibrated flasks where they were diluted with 10 ml of dichloromethane . One microlitre of standard and sample solutions was injected, and the chromatography was carried out at 60 °C and with pressure-programming from 100–450 atm. A flame ionization detector was used in the determination. The proposed method is rapid and there is no interference from other constituents of the samples, providing analytical recoveries of 94–102%. Use of CO2 as both extractant and mobile phase reduces the use of organic solvents to only the small volume necessary to dilute the extracts.


References

  1. M. J. Deutsch, Official Methods of Analysis, Association of Official Analytical Chemists, VA, 1990, 1070–1079 Search PubMed.
  2. United States Pharmacopeial Convention, USP, Rockville, 1985 Search PubMed.
  3. A. Deschuytere and H. Deelstra, Fresenius' J. Anal. Chem., 1986, 324, 1 CrossRef CAS.
  4. M. Amin, J. Liq. Chromatogr., 1988, 11, 1335 CAS.
  5. M. Careri, M. T. Lugari, A. Mangia, P. Manini and S. Spagnoli, Fresenius' J. Anal. Chem., 1995, 351, 768 CrossRef CAS.
  6. K. E. Savolainen, K. M. Pynnonen, S. P. Lapinjoki and M. T. Vidgren, J. Pharm. Sci., 1988, 77, 802 CAS.
  7. W. Ying, Z. Li, D. Xue, N. Chen and Y. Chen, Sepu, 1989, 7, 298 Search PubMed.
  8. K. D. Bartle and T. Clifford, Analysis Europa, 1995, 4, 14 Search PubMed.
  9. T. L. Chester, J. D. Pinkston and D. E. Raynie, Anal. Chem., 1994, 66, 106R CAS.
  10. M. D. Luque de Castro, M. Valcárcel and M. T. Tena, Analytical Supercritical Fluid Extraction, Springer-Verlag, London, 1994 Search PubMed.
  11. L. Taylor, Supercritical Fluid Extraction, Wiley, New York, 1995 Search PubMed.
  12. L. Karlsson, A. Torstensson and L. T. Taylor, J. Pharm. Biomed. Anal., 1997, 15, 601 CrossRef CAS.
  13. D. C. Messer, L. T. Taylor, W. N. Moore and W. E. Weiser, Ther. Drug. Monit., 1993, 15, 581 CAS.
  14. A. Salvador, M. A. Jaime, G. Becerra and M. de la Guardia, Fresenius' J. Anal. Chem, 1996, 356, 109 CAS.
  15. C. M. White, D. R. Gere, D. Boyer, F. Pacholec and L. K. Wong, J. High Resolut. Chromatogr. Chromatogr. Commun., 1988, 11, 94 Search PubMed.
  16. E. Ibáñez, J. Tabera, G. Reglero and M. Herraiz, J. Agric. Food Chem., 1995, 43, 2667 CrossRef CAS.
  17. S. Scalia, G. Ruberto and F. Bonina, J. Pharm Sci., 1995, 84, 433 CAS.
  18. M. Valcarcel, M. D. Luque de Castro and M. T. Tena, Ann. Chim., 1995, 85, 1 CAS.
  19. J. R. Williams and E. D. Morgan, Chromatogr. Anal., 1995, 38, 9 Search PubMed.
Click here to see how this site uses Cookies. View our privacy policy here.