Thickness shear mode acoustic assay for plasminogen activators

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G. L. Hayward, J. M. Scharer, R. L. Dutton, Z. Zhang and M. Moo Young


Abstract

A micro version of the fibrin plate assay based on acoustic viscosity measurements is presented. The clot dissolution time was correlated with the amount of plasminogen activator in the samples assayed. The standard error observed with streptokinase (27%) was comparable with that of the rapid fibrin plate assay while a higher standard error was observed with tissue plasminogen activator (59%). The higher error was attributed to diffusion effects and to acoustic compression wave resonances. The method is rapid, sensitive and very small quantities of reagents are required.


References

  1. S. A. Rouf, M. Moo-Young and Y. Chisti, Biotechnol. Adv., 1996, 14, 239 CrossRef CAS.
  2. R. V. Datar, T. Cartwright and C.-G. Rosen, Biotechnology, 1993, 11, 349 Search PubMed.
  3. C. Bode, M. S. Runge and R. W. Smalling, Eur. Heart J., 1996, 17, (Suppl. E), 55.
  4. O. Matsuo, K. Kato, C. Matsuo and T. Matsuo, Anal. Biochem., 1983, 135, 58 CrossRef CAS.
  5. A. A. Lin, R. Kimura and W. M. Miller, Biotech. Bioeng., 1993, 42, 339 CrossRef CAS.
  6. N. A. Marsh and P. J. Gaffney, Thromb. Haemostasis, 1977, 38, 545 Search PubMed.
  7. H. Muramatsu, E. Tamiya, M. Suzuki and I. Karube, Anal. Chim. Acta, 1988, 215, 91 CrossRef CAS.
  8. H. Muramatsu, E. Tamiya, M. Suzuki and I. Karube, Anal. Chim. Acta, 1989, 217, 321 CrossRef.
  9. T. Nomura and M. Okuhara, Anal. Chim. Acta, 1982, 142, 2814 CrossRef CAS.
  10. R. L. Simpson, PhD Thesis, University of Washington, 1985.
  11. G. L. Hayward, Anal. Chim. Acta, 1992, 264, 23 CrossRef CAS.
  12. Y. Takada and A. Takada, in Fibrinogen, Thrombosis, Coagulation and Fibrinolysis, ed. Liu, C. Y., and Chien, S., Plenum, New York, 1989, 223–234 Search PubMed.
  13. G. L. Hayward and G.-Z. Chu, Anal. Chim. Acta, 1994, 288, 179 CrossRef CAS.
  14. K. K. Kanazawa and J. G. Gordon, Anal. Chim. Acta, 1985, 175, 99 CrossRef CAS.
  15. S. M. Reddy, J. P. Jones and T. J. Lewis, Faraday Discuss., 1997, 107 Search PubMed in the press.
  16. A. Savitzky and M. J. E. Golay, Anal. Chem., 1964, 36, 1627 CrossRef CAS.
  17. J. C. Miller and J. N. Miller, Statistics for Analytical Chemistry, Ellis Horwood, Chichester, 2nd edn., 1988 Search PubMed.
  18. M. Gronow and R. Bliem, Trends Biotechnol., 1983, 1, 26 CrossRef CAS.
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