A radio-iodinated abscisic acid photoaffinity probe

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Andrew D. Kohler, Michael H. Beale, Ruth Rollason, D. H. Paul Barratt, Mervyn J. Lewis, R. M. Van der Meulen and Mei Wang


Abstract

The construction of a photoaffinity probe based on abscisic acid is described. A strategy based on C-4′-tethering of aromatic hydrazides led to abscisic acid 4′-(3″-azido-4″ -hydroxybenzoylhydrazide). This compound is of moderate biological activity in cereal aleurone assays and was readily iodinated with chloramine T and sodium iodide, conditions that were used for the preparation of the [125I]-labelled compound in high radiochemical yield.


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