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DOI: 10.1039/A704079B (Paper) Reference Section for: J. Anal. At. Spectrom., 1997, 12, 919-923

Measurements of44Ca:43Ca and 42Ca:43Ca Isotope Ratios in Urine Using High Resolution Inductively Coupled Plasma Mass Spectrometry

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STEFAN STÜRUP, MARIANNE HANSEN and CHRISTIAN MØLGAARD

Abstract

A method is described for the measurement of calcium isotopic ratios (42Ca+:43Ca+ and 44Ca+:43Ca+) in human urine using high resolution inductively coupled plasma mass spectrometry (HR-ICP-MS). Relative standard deviations (RSD) of 0.33 and 0.41% were found for 44Ca+:43Ca+ and 42Ca+:43Ca+, respectively. Using a mass spectrometric resolution setting of 4000, the calcium peaks were resolved from interfering polyatomic ions (40Ar2H+ and 40ArH2+ on 42Ca+ and 28Si16O+ and 12C16O2+ on 44Ca+). Interferences from Sr2+, which cannot be resolved even using high resolution, were corrected for mathematically using the 43.5Sr2+ peak. The sample preparation step consisted of a simple 50-fold dilution of the urine sample with 0.14 M HNO3, which ensured a relatively high sample throughput of 6 samples per hour.

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