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DOI: 10.1039/A701695F (Paper) Reference Section for: J. Anal. At. Spectrom., 1997, 12, 1041-1046

Determination of Selenomethionine in Wheat Samples: Comparison of Gas Chromatography-Microwave-induced Plasma Atomic Emission Spectrometry, Gas Chromatography-Flame Photometric Detection and Gas Chromatography–Mass Spectrometry

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M. BEATRIZ DE LA CALLE-GUNTIÑAS, CLAUDIA BRUNORI, ROSSANA SCERBO, SALVATORE CHIAVARINI, PHILIPPE QUEVAUVILLER, FREDDY ADAMS and ROBERTO MORABITO

Abstract

A method is described for the selective determination of selenomethionine in wheat samples. The method is based on three steps prior to the determination: (1) hydrolysis of the proteins with tetramethylammonium hydroxide, (2) clean-up on an aluminium oxide column (acid form), (3) derivatization to convert selenomethionine into a compound suitable for GC. The derivatization reaction consists in esterification of the carboxylic acid group with propan-2-ol in 4 mol l-1 HCl medium and subsequent acylation of the amino group with heptafluorobutyric anhydride. The parameters affecting the efficiency of both reactions were optimized, as well as the esterification medium. Other esterification and acylation reagents were also tested. Three different detection techniques were evaluated using selenomethionine standard solutions: FPD, MIP-AES and MS. Electrothermal AAS was also tested, but proved to be unreliable. MS was selected as the best option.

References

  1. NAS, Medical and Biological Effects of Environmental Pollutants–Selenium, National Academy of Sciences, Washington, DC, 1976 Search PubMed.
  2. A. T. Diplock, Philos. Trans. R. Soc. London Ser. B, 1981, 294, 105 Search PubMed.
  3. V. R. Young, N. Engl. J. Med., 1981, 304, 1228 CAS.
  4. L. N. Vernie, Biochim. Biophys. Acta, 1984, 738, 203 CAS.
  5. A. L. Moxon and O. E. Olson, in: Selenium, ed. Zingaro, R. A., and Cooper, W. C., Van Nostrand Reinhold, New York, 1974, p. 675 Search PubMed.
  6. L. Fisbein, in: Metals and their Compounds in the Environment. Occurrence, Analysis, and Biological Relevance, ed. Merian, E., VCH, Weinheim, 1991, p. 1153 Search PubMed.
  7. L. D. Koller and J. H. Exon, Can. J. Vet. Res., 1986, 50, 297 Search PubMed.
  8. NAS, Selenium in Nutrition, National Academy of Sciences, Washington, DC, 1983 Search PubMed.
  9. J. Glover, O. Levander, J. Parizeck, G. F. Nordberg and V. Vouk, in: Handbook on the Toxicology of Metals, ed. Froberg, L., Nordberg, G. F., and Vouk, V. B., Elsevier, North Holland Biochemical Press, Amsterdam, 1979, p. 555 Search PubMed.
  10. M. A. Beilstein and P. D. Whanger, J. Nutr., 1986, 116, 1711 Search PubMed.
  11. R. J. Kraus, S. J. Foster and H. E. Ganther, Biochemistry, 1983, 22, 5853 CrossRef CAS.
  12. H. E. Ganther and R. J. Kraus, Anal. Biochem., 1984, 138, 396 CrossRef.
  13. H. E. Ganther, J. Am. Coll. Toxicol., 1986, 5, 1 Search PubMed.
  14. M. M. Abrams and R. G. Burau, Commun. Soil Sci. Plant Anal., 1989, 20, 221 Search PubMed.
  15. N. R. Bottino, C. H. Banks, K. J. Irgolin, P. Micks, A. E. Wheeler and R. A. Zingaro, Phytochemistry, 1984, 23, 2445 CrossRef CAS.
  16. R. Muñoz Olivas, O. F. X. Donard, C. Cámara and Ph. Quevauviller, Anal. Chim. Acta, 1994, 286, 357 CrossRef.
  17. M. B. de la Calle-Guntiñas, M. Ceulemans, C. Witte, R. Lobinski and F. C. Adams, Mikrochim. Acta, 1995, 120, 73 CAS.
  18. D. Tanzer and K. G. Heumann, Atmos. Environ., 1990, 24A, 3099 CAS.
  19. X. Dauchy, M. Potin-Gautier, A. Astruc and M. Astruc, Fresenius' J. Anal. Chem., 1994, 348, 792 CAS.
  20. W. M. R. Dirkx, R. Lobinski and F. C. Adams, Anal. Sci., 1993, 9, 273 CAS.
  21. X. W. Lou, X. L. Liu, D. Q. Zhu and L. M. Zhou, J. Chromatogr., 1991, 626, 231 CrossRef CAS.
  22. V. Fabian, M. Morvai, M. Pinter-Szakaes and I. Molnar-Perl, J. Chromatogr., 1991, 553, 87 CrossRef CAS.
  23. J. March, Advanced Organic Chemistry. Reactions, Mechanisms, and Structure, Wiley-Interscience, New York, 1985 Search PubMed.
  24. R. Scerbo, M. B. de la Calle-Guntiñas, C. Brunori, S. Chiavarini and R. Morabito, Quím. Anal., in the press Search PubMed.
  25. M. B. de la Calle-Guntiñas, R. Łobiński and F. C. Adams, J. Anal. At. Spectrom., 1995, 10, 111 RSC.
  26. D. Liu, J. High Resolut. Chromatogr. Chromatogr. Commun., 1989, 12, 239 Search PubMed.
  27. K. Yasumoto, T. Suzuki and M. Yoshida, J. Agric. Food Chem., 1988, 36, 463 CrossRef CAS.
  28. M. Ceulemans, C. Witte, R. Łobinski and F. C. Adams, Appl. Organomet. Chem., 1994, 8, 451 CrossRef CAS.
  29. C. M. Tseng, A. de Diego, F. M. Martin, D. Amouroux and O. F. X. Donard, J. Anal. At. Spectrom., in the press Search PubMed.
  30. C. Brunori, M. B. de la Calle-Guntiñas, M. Angelone and R. Morabito, unpublished work.
  31. A. Pirini, L. S. Conte, O. Francioso and G. Lereker, J. High Resolut. Chromatogr., 1992, 15, 165 CAS.
  32. H. Brueckner and M. Luepke, Chromatographia, 1991, 31(3–4), 123 CAS.
  33. A. M. P. Vasconcelos and H. J. Chaves das Neves, J. High Resolut. Chromatogr., 1990, 13, 494 CAS.
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