Folding of long DNA chains in the presence of distearyldimethylammonium bromide and unfolding induced by neutral liposomes

Abstract

The interaction between large duplex T4DNA (166 kbp) and a synthetic dialkyl cationic lipid, distearyldimethylammonium bromide (D₁₈DAB), was studied using fluorescence microscopy for single-chain observation. The dependence of the higher-order structure of single T4DNAs on the surfactant concentration was evaluated, starting at extremely low values (1.0×10^-8M). We found that individual T4DNA chains undergo a marked discrete transition between elongated coil and compact globule states, and that there is a very wide region of coexistence (about two orders of magnitude of the surfactant concentration) for the coiled and globular T4DNAs. We propose a simple theoretical model for assessing the relationship between the binding equilibrium and the coil–globule transition. In addition, we found that liposomes composed of neutral phospholipids induce unfolding of DNA compacted by a cationic surfactant.

References

1. S. M. Mel'nikov, V. G. Sergeyev and K. Yoshikawa, J. Am. Chem. Soc., 1995, 117, 2401.
2. S. M. Mel'nikov, V. G. Sergeyev and K. Yoshikawa, J. Am. Chem. Soc., 1995, 117, 9951.
3. M. Yanagida, Y. Hiraoka and I. Katsura, Cold Spring Harbor Symp. Quant. Biol., 1983, 47, 177.
4. C. Bustamante, Annu. Rev. Biophys. Biophys. Chem., 1991, 20, 415.
5. K. Minagawa, Y. Matsuzawa, K. Yoshikawa, M. Matsumoto and M. Doi, FEBS Lett., 1991, 295, 67.
6. K. Minagawa, Y. Matsuzawa, K. Yoshikawa, A. R. Khokhlov and M. Doi, Biopolymers, 1994, 34, 555.
7. T. T. Perkins, S. R. Quake, D. E. Smith and S. Chu, Science, 1994, 264, 822.
8. T. T. Perkins, D. E. Smith and S. Chu, Science, 1994, 264, 819.
9. K. Ijiro and Y. Okahata, J. Chem. Soc., Chem. Commun., 1992, 1339.
10. Y. Okahata, Y. Matsuzaki and K. Ijiro, Sensors Actuators B, 1993, 13–14, 380.
11. Y. Okahata, K. Ijiro and Y. Matsuzaki, Langmuir, 1993, 9, 19.
12. K. Hayakawa, J. P. Santerre and J. C. T. Kwak, Biophys. Chem., 1983, 17, 175.
13. K. Shirahama, T. Masaki and K. Takashima, in Microdomains in Polymer Solutions, ed. P. Dubin, Plenum, New York, 1986, p. 299.
14. K. Shirahama, K. Takashima and N. Takisawa, Bull. Chem. Soc. Jpn., 1987, 60, 43.
15. M. Lapidot and A. Loyter, Exp. Cell Res., 1990, 189, 241.
16. J-P. Behr, Acc. Chem. Res., 1993, 26, 274.
17. P. L. Felgner, T. R. Gadek, M. Holm, R. Roman, H. W. Chan, M. Wenz, J. P. Northrop, G. M. Ringold and M. Danielsen, Proc. Natl. Acad. Sci. USA, 1987, 84, 7413.
18. X. Zhou, A. L. Klibanov and L. Huang, Biochim. Biophys. Acta, 1991, 1065, 8.
19. H. Gershon, R. Ghirlando, S. B. Guttman and A. Minsky, Biochemistry, 1993, 32, 7143.
20. J-S. Remy, C. Sirlin, P. Vierling and J-P. Behr, Bioconjugate Chem., 1994, 5, 647.
21. B. Sternberg, F. L. Sorgi and L. Huang, FEBS Lett., 1994, 356, 361.
22. X. Zhou and L. Huang, Biochim. Biophys. Acta, 1994, 1189, 195.
23. P. L. Felgner and G. M. Ringold, Nature (London), 1989, 337, 387.
24. D. L. Reimer, Y-P. Zhang, S. Kong, J. J. Wheeler, R. W. Graham and M. B. Bally, Biochemistry, 1995, 34, 12877.
25. H. Hauzer, Biochim. Biophys. Acta, 1984, 772, 37.
26. D. D. Lasic, J. Colloid Interface Sci., 1988, 124, 428.
27. N. Kumazawa, Yu. S. Mel'nikova and K. Yoshikawa, Makromol. Chem., Macromol. Symp., 1996, 106, 219.
28. K. Yoshikawa, Y. Matsuzawa, K. Minagawa, M. Doi and M. Matsumoto, Biochem. Biophys. Res. Commun., 1992, 188, 1274.
29. D. D. Lasic, Biochem. J., 1988, 256, 1.
30. Y. Matsuzawa and K. Yoshikawa, Nucleosides Nucleotides, 1994, 13, 1415.
31. G. Schwarz, Eur. J. Biochem., 1970, 12, 442.
32. K. S. Schmitz and J. M. Schurr, Biopolymers, 1970, 9, 697.
33. I. Satake and J. T. Yang, Biopolymers, 1976, 15, 2263.
34. V. A. Efremov, A. R. Khokhlov and Yu. V. Shikina, Polym. Sci. USSR (Engl. Transl.), 1992, 34, 484.
35. B. Lindman and K. Thalberg, in Interactions of Surfactants with Polymers and Proteins, ed. E. D. Goddard and K. P. Ananthapadmanabhan, CRC Press, Boca Raton, FL, 1993, p. 203.
36. H. Farhood, N. Serbina and L. Huang, Biochim. Biophys. Acta, 1995, 1235, 289.
37. C. Nicolau and A. Cudd, Crit. Rev. Ther. Drug Carr. Syst. A, 1989, 6, 239.
38. R. Leventis and J. R. Silvius, Biochim. Biophys. Acta, 1990, 1023, 124.
39. P. Pinnaduwage, L. Schmitt and L. Huang, Biochim. Biophys. Acta, 1989, 985, 33.
40. H. Hotani, J. Mol. Biol., 1984, 178, 113.
41. F. M. Menger and N. Balachander, J. Am. Chem. Soc., 1992, 114, 5862.
42. F. M. Menger and K. Gabrielson, J. Am. Chem. Soc., 1994, 116, 1567.
43. E. B. Abuin and J. C. Scaiano, J. Am. Chem. Soc., 1984, 106, 6274.
44. E. D. Goddard, Colloids Surf., 1986, 19, 301.
45. B. Lindman and G. Karlström, in The Structure, Dynamics and Equilibrium Properties of Colloidal Systems, ed. D. M. Bloor and E. Wyn-Jones, Kluwer Academic Publishers, Dordrecht, 1990, p. 131.
46. M. N. Jones, Chem. Soc. Rev., 1992, 21, 127.