Jian-Zhong Wu, Bao-Hui Ye, Lei Wang, Liang-Nian Ji, Jian-Ying Zhou, Run-Hua Li and Zhong-Yuan Zhou
Imidazo[4,5-f]1,10-phenanthroline (ip),
2-phenylimidazo[4,5-f][1,10]phenanthroline (pip) and
their (bipy)2Ru2+ complexes
(bipy = 2,2′-bipyridine) have been synthesized and
characterized. The oxidation potentials of
[Ru(bipy)2(ip)]2+ and
[Ru(bipy)2(pip)]2+ were found to be 1.254 and
1.284 V vs. saturated calomel electrode respectively; the
reduction of ip and pip appears to be irreversible at ca.
-0.85 V. The photophysical properties of the complexes were
perturbed in the presence of calf thymus DNA. The distinct changes
including hypo- or hyper-chromicity at different UV/VIS absorption
bands, enhancements of integrated emission intensity and excited-state
lifetime, and efficiency of emission quenching by
[Fe(CN)6]4- all indicate the stronger
binding affinity to DNA of [Ru(bipy)2(pip)]2+ over
that of [Ru(bipy)2(ip)]2+, consistent with the
greater planar area and extended π system of the pip ligand. The
luminescence of the complexes showed monoexponential decay at any
[DNA]∶[Ru] ratio. The circular dichroism signals of the dialysates
of the racemic complexes against calf thymus DNA suggest that the
complexes bind to the DNA with enantioselectivity favouring the Δ
isomers. These phenomena all suggest that the complexes bind through
intercalation of ip or pip into base pairs. The crystal structure of
[Ru(bipy)2(ip)][ClO4]2·H2
O was determined; it contains the planar bidentate ligand ip and
two twisted bipy ligands with torsional angles between each bipyridine
ring pair of 5.7 and 8.6°.