Dissecting the effects of cooperativity on the stabilisation of a de novo designed three stranded anti-parallel β-sheet

Abstract

Cooperative effects between two sets of weak interactions at different hydrogen bonded interfaces are shown to stabilise a de novo designed three-stranded anti-parallel β-sheet: NMR and CD (circular dichroism) spectroscopy have been used to compare and contrast the stability of the β-sheet (residues 1–24) and the isolated C-terminal β-hairpin (residues 9–24).

References

1. D. H. Williams and M. S. Westwell, Chem. Biol., 1996, 3, 695.
2. M. S. Westwell, M. S. Searle and D. H. Williams, J. Mol. Recognit., 1996, 9, 88.
3. D. H. Williams, M. S. Searle, M. S. Westwell, J. P. Mackay, P. Groves and D. A. Beauregard, Chemtracts, 1994, 7, 133.
4. J. P. Mackay, U. Gerhard, D. A. Beauregard, M. S. Westwell, M. S. Searle and D. H. Williams, J. Am. Chem. Soc., 1994, 116, 4581.
5. M. F. Perutz, Proc. R. Soc. London, Ser. B, 1980, 208, 135.
6. Q. X. Hua, W. H. Jia, B. H. Frank and M. A. Weiss, J. Mol. Biol., 1993, 230, 387.
7. J. Monod, J. Wyman and J. P. Changeux, J. Mol. Biol., 1965, 12, 88.
8. F. J. Blanco, G. Rivas and L. Serrano, Nat. Struct. Biol., 1994, 1, 584; F. J. Blanco, M. A. Jimenez, J. Herranz, M. Rico, J. Santoro and J. L. Nieto, J. Am. Chem. Soc., 1993, 115, 5887; M. S. Searle, D. H. Williams and L. C. Packman, Nat. Struct. Biol., 1995, 2, 999; E. de Alba, M. A. Jimenez, M. Rico and J. L. Nieto, Folding Des., 1996, 1, 133; E. de Alba, M. A. Jimenez and M. Rico, J. Am. Chem. Soc., 1997, 119, 175; T. S. Haque and S. H. Gellman, J. Am. Chem. Soc., 1997, 119, 2303; A. J. Maynard and M. S. Searle, Chem. Commun., 1997, 1297.
9. W. C. Johnson, Annu. Rev. Biophys. Biophys. Chem., 1988, 17, 145.
10. J. T. Yang, C. C. Wu and H. M. Martinez, Methods Enzymol., 1986, 130, 208.
11. D. S. Wishart, B. D. Sykes and F. M. Richards, J. Mol. Biol., 1991, 222, 311.
12. NMR chemical shifts and line widths were shown to be independent of concentration in the range 5 mM to 50 µM.
13. M. S. Searle, M. S. Westwell and D. H. Williams, J. Chem. Soc., Perkin Trans. 2, 1995, 141.
14. M. S. Searle, G. J. Sharman, P. Groves, B. Benhamu, D. A. Beauregard, M. S. Westwell, R. J. Dancer, A. J. Maguire, A. C. Try and D. H. Williams, J. Chem. Soc., Perkin Trans. 1, 1996, 23, 2781.
15. H. Guo and M. Karplus, J. Phys. Chem., 1994, 98, 7104.
16. T. Arakawa and D. Goddette, Arch. Biochem. Biophys., 1985, 240, 21.
17. J. W. Nelson and N. R. Kallenbach, Proteins, 1986, 1, 211.
18. F. D. Sonnichsen, J. E. Van Eyk, R. S. Hodges and B. D. Sykes, Biochemistry, 1992, 31, 8790.
19. R. W. Storrs, D. Truckses and D. E. Wemmer, Biopolymers, 1992, 32, 1695.