Dissecting the effects of cooperativity on the stabilisation of a de novo designed three stranded anti-parallel β-sheet

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Gary J. Sharman


Abstract

Cooperative effects between two sets of weak interactions at different hydrogen bonded interfaces are shown to stabilise a de novo designed three-stranded anti-parallel β-sheet: NMR and CD (circular dichroism) spectroscopy have been used to compare and contrast the stability of the β-sheet (residues 1–24) and the isolated C-terminal β-hairpin (residues 9–24).


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