Time-resolved solid-state REDOR-edited NMR detection of a transient enzyme–intermediate

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Dan J. Mitchell, David L. Jakeman, Tatyana I. Igumenova, Wendy A. Shuttleworth, Keith D. Miller and Jeremy N. S. Evans


Abstract

Time-resolved solid-state rotational echo double resonance-edited NMR has been used to detect transient enzyme–intermediates containing two dipolar coupled spins that are unique to the intermediate, so that even resonances buried in the natural abundance background can be detected.


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