A novel acridone derivative for the fluorescence tagging and mass spectrometric sequencing of peptides

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Thomas Faller, Kathryn Hutton, George Okafo, Andy Gribble, Patrick Camilleri and David E. Games


Abstract

An acridone derivative containing an activated carboxylic group is highly reactive towards primary amine groups, and allows selective analysis either using absorbance at a wavelength of 410 nm or fluorescence at excitation and emission wavelengths of 400 and 418 nm, respectively; MS–MS analysis of peptides tagged with this reagent gives fragmentation patterns which are usually considerably more intense than background.


References

  1. K. R. Anumula, R. P. Schulz and N. Back, Peptides, 1992, 13, 663 CrossRef CAS.
  2. C. G. Bradshaw, K. Ceszkowski, G. Turcatti, I. J. Beresford and A. Chollet, J. Med. Chem., 1994, 37, 1991 CrossRef CAS.
  3. N. W. Bunnett, P. F. Dazin, D. G. Payan and E. F. Grady, Peptides, 1995, 16, 733 CrossRef CAS.
  4. G. Panigrahi, B. Zhao, J. J. Krepinsky and P. Sadowski, J. Am. Chem. Soc., 1996, 118, 12004 CrossRef CAS.
  5. M. Greenaway, G. N. Okafo, P. Camilleri and D. Dhanak, J. Chem. Soc., Chem. Commun., 1994, 1691 RSC.
  6. G. Okafo, L. Burrow, S. A. Carr, G. D. Roberts, W. Johnson and P. Camilleri, Anal. Chem., 1996, 68, 4424 CrossRef CAS.
  7. J. L. Reymond, T. Koch, J. Schroer and E. Tierney, Proc. Natl. Acad. Sci. USA, 1996, 93, 4251 CrossRef CAS.
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