DOI: 10.1039/A608362E
(Paper)
Reference Section for: Chem. Commun., 1997, 713-714
A new approach to identifying substrate binding sites on isolated FeMo-cofactor of nitrogenase
(Note: The full text of this document is currently only available in the PDF Version )
Karin L. C. Grönberg, Carol A. Gormal, Barry E. Smith and Richard A. Henderson
Abstract
A kinetic method is described which is capable of detecting the binding of molecules and ions such as CN-, N3-, ButNC, imidazole and H+ to the isolated FeMo-cofactor of nitrogenase, and indicates where on the cofactor these species bind.
References
- T. R. Hawkes, P. A. McLean and B. E. Smith, Biochem. J., 1984, 217, 317 CAS.
- B. K. Burgess, Chem. Rev., 1990, 90, 1377 CrossRef CAS.
- M. K. Chan, J. Kim and D. C. Rees, Science, 1993, 260, 792 CrossRef CAS.
- Related effects are observed when small molecules (such as halide, CO, N3– and CN-) bind to synthetic clusters such as [{MoFe3S4(SEt)3}2(µ-SEt)3]3–; R. A. Henderson and K. E. Oglieve, J. Chem. Soc., Dalton Trans., 1993, 1473 Search PubMed and references therein.
- A. J. M. Richards, D. J. Lowe, R. L. Richards, A. J. Thomson and B. E. Smith, Biochem. J., 1994, 297, 373 CAS.
- H. I. Liu, A. Filipponi, N. Gavini, B. K. Burgess, B. Hedman, A. Di Cicco, C. R. Natoli and K. O. Hodgson, J. Am. Chem. Soc., 1994, 116, 2418 CrossRef CAS.
- K. L. C. Grönberg and R. A. Henderson, J. Chem. Soc., Dalton Trans., 1996, 3667 RSC and references therein.
- S. D. Conradson, B. K. Burgess, S. A. Vaughn, A. L. Roe, B. Hedman, K. O. Hodgson and R. H. Holm, J. Biol. Chem., 1989, 264, 15 967 CAS and references therein.
- A. J. M. Richards, PhD Thesis, University of East Anglia, 1986.
- F. A. Schultz, B. J. Feldman, S. F. Gheller and W. E. Newton, Inorg. Chim. Acta, 1990, 170, 115 CrossRef CAS; W. E. Newton, S. F. Gheller, B. J. Feldman, W. R. Dunham and F. A. Schultz, J. Biol. Chem., 1989, 264, 1924 CAS.