Bioelectrochemical Determination of Citric Acid in Real Samples Using a Fully Automated Flow Injection Manifold

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Mamas I. Prodromidis, Stella M. Tzouwara-Karayanni, Miltiades I. Karayannis, Mamas I. Prodromidis and Pankaj M. Vadgama


Abstract

An enzymic method for the determination of citric acid in fruits, juices and sport drinks is proposed. The method is based on the action of the enzymes citrate lyase, oxaloacetate decarboxylase and pyruvate oxidase, which convert citric acid into H2O2 with the latter being monitored amperometrically with a H2O2 probe. The enzymes pyruvate oxidase and oxaloacetate decarboxylase were immobilized. A multi-membrane system, consisting of a cellulose acetate membrane for the elimination of interferants, an enzymic membrane and a protective polycarbonate membrane were placed on a Pt electrode and used with a fully automated flow injection manifold. Several parameters were optimized, resulting in a readily constructed and reproducible biosensor. Interference from various compounds present in real samples was minimized. Calibration graphs were linear over the range 0.01–0.9 mM pyruvate, 0.015–0.6 mM oxaloacetate and 0.015–0.5 mM citrate. The throughput was 30 samples h1 with an RSD of 1.0% (n = 8); the mean relative error was 2.4% compared with a standard method. The recovery was 96–104%. A 8–10% loss of the initial activity of the sensor was observed after 100–120 injections.


References

  1. W. H. Gardner, in Handbook of Food Additives, ed. Furioc, T. E., CRC Press; Cleveland, OH, 2nd edn., 1972, pp. 242–246 Search PubMed.
  2. G. K. Murthy, E. B. Masurowsky, J. E. Campbell and L. F. Edmondson, US Pat. 3 020 161, 1962 Search PubMed; Chem. Abstr., 1962, 56, P14682g Search PubMed.
  3. Y. Kasai, T. Tanimura and Z. Tamura, Anal. Chem., 1975, 47, 34 CrossRef CAS.
  4. E. D. Coppola, E. C. Conrad and R. Cotter, J. Assoc. Off. Anal. Chem., 1978, 61, 1490 Search PubMed.
  5. P. Bocek, K. Lekova, M. Deml and J. Janak, J. Chromatogr., 1976, 117, 97 CrossRef CAS.
  6. K. Matsumoto, K. Ishida, T. Nomura and Y. Osajima, Agric. Biol. Chem., 1984, 48, 2211 CAS.
  7. L. Dunemann, Anal. Chim. Acta, 1989, 221, 19 CrossRef CAS.
  8. L. B. Spector, in The Enzymes, ed. Boyer. P. D., Academic Press, New York, 3rd edn., 1975, vol. VIII, 378 Search PubMed.
  9. H. Moellering and W. Gruber, Anal. Biochem., 1966, 17, 369 CrossRef CAS.
  10. M. Plantá, F. Lazaro, R. Puchades and A. Maquieira, Analyst, 1993, 118, 1193 RSC.
  11. K. Hasebe, S. Hikima, T. Kakizaki and H. Yoshida, Fresenius' Z. Anal. Chem., 1989, 333, 19 Search PubMed.
  12. S. Hikima, K. Hasebe and M. Taga, Electroanalysis, 1992, 4, 801 CAS.
  13. K. Matsumoto, T. Tsukatani and Y. Okajima, Electroanalysis, 1995, 7, 527 CAS.
  14. N. Gajovic, A. Warsinke and F. W. Scheller, J. Chem. Tech. Biotechnol., 1995, 63, 337 Search PubMed.
  15. S. Dagley, in Methods in Enzymology, ed. Lowestein, I. M., Academic Press, New York, 1969, vol. XIII, ch. 67 Search PubMed.
  16. M. I. Prodromidis, A. B. Tsibiris and M. I. Karayannis, J. Autom. Chem., 1995, 17, 187 Search PubMed.
  17. Enzymatic Analysis. A Practical Guide, ed. Passonneau, J. V., and Lowry, H. O., Humana Press, Clifton, NJ, 1993 Search PubMed.
  18. C. H. Assoland-Vinet and P. R. Coulet, Anal. Lett., 1986, 19, 875.
  19. F. Mizutani, K. Tsuda, I. Karube, S. Suzuki and K. Matsumoto, Anal. Chim. Acta, 1980, 118, 65 CrossRef CAS.
  20. M. Mascini, M. Iannello and G. Palleschi, Anal. Chim. Acta, 1983, 146, 135 CrossRef CAS.
  21. G. Palleschi, M. A. Nabi Rahni, G. J. Lubrano, J. H. Ngwainbi and G. G. Guilbault, Anal. Biochem., 1986, 159, 114 CrossRef CAS.
  22. W. J. Albery, P. N. Bartlett and D. H. Craston, J. Electroanal. Chem., 1985, 194, 223 CrossRef CAS.
  23. K. Kihara, E. Yasukawa and S. Hirose, Anal. Chem., 1984, 56, 1876 CrossRef CAS.
  24. L. P. Hager and F. Lipmann, in Methods in Enzymology, ed. Colowick, S. P., and Kaplan, N. O., Academic Press, New York, 1955, vol. I, ch. 75 Search PubMed.
  25. M. S. M. Jetten and A. J. Sinskey, Antonie van Leeuwenhoek Int. J., 1995, 67, 221 Search PubMed; Chem. Abstr., 123; 283169 Search PubMed.
  26. M. A. Joslyn, in Methods In Food Analysis, Academic Press; New York, 1970, ch. XIV, p. 408 Search PubMed.
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