Catalytic Oxidation of Uric Acid at the Polyglycine Chemically Modified Electrode and its Trace Determination

(Note: The full text of this document is currently only available in the PDF Version )

Ai-min Yu, Hai-li Zhang and Hong-yuan Chen


Abstract

Cyclic voltammetry was undertaken to investigate the electrochemical behavior of uric acid at a polyglycine modified electrode. The modified electrode shows catalytic ability for the oxidation of uric acid, reducing the overpotential by 250 mV in pH 7.0 phosphate buffer solution. The enhanced voltammetric response can be used to determine uric acid. The linear range is between 5.0 × 10-8 and 4.5 × 10-6M with a detection limit as low as 5.0 × 10-9M. The relative standard deviation is 1.4% (8 runs) at a concentration of 50 µM uric acid. The catalytic effect of the modified electrode resulted in the voltammetric resolution of the overlapping of uric acid and ascorbic acid. This allows the simultaneous detection of uric acid and ascorbic acid in the same sample.


References

  1. H. A. Harper, Review of Physiological Chemistry, Lange Medical Publications, CA, 16th edn., 1977, p. 406 Search PubMed.
  2. T. Yao, Y. Taniguchi, T. Wasa and S. Mushs, Bull. Chem. Soc. Jpn., 1978, 51, 2937 CAS.
  3. G. Park, R. N. Adams and W. R. White, Anal. Lett., 1972, 5, 887 CAS.
  4. J. Wang and B. A. Freiha, Bioelectrochem. Bioenerg., 1984, 12, 225 CrossRef CAS.
  5. M. A. T. Gilmartin and J. P. Hart, Analyst, 1992, 117, 1299 RSC.
  6. X.-H. Cai, K. Kalcher, C. Neuhold and B. Ogorevc, Talanta, 1994, 41, 407 CrossRef CAS.
  7. T. Tatsuma and T. Watanabe, Anal. Chim. Acta, 1991, 242, 85 CrossRef CAS.
  8. F. E. Keedy and B. Vadgama, Biosens. Bioelectron., 1991, 6, 491 CrossRef CAS.
  9. M. B. Gelbert and D. J. Curran, Anal. Chem., 1986, 58, 1028 CAS.
  10. E. W. Kristensen, W. G. Kuhr and R. M. Wrightman, Anal. Chem., 1987, 591, 752.
  11. M. J. Rocheleau and W. C. Purdy, Electroanalysis, 1991, 3, 935 CAS.
Click here to see how this site uses Cookies. View our privacy policy here.