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DOI: 10.1039/A606881B (Paper) Reference Section for: Analyst, 1997, 122, 139-142

Spectrofluorimetric Determination of Vitamin K3 by a Solid-phase Zinc Reactor Immobilized in a Flow Injection Assembly

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I. Gil Torró, J. V. García Mateo and J. Martínez Calatayud

Abstract

The spectrofluorimetric determination of vitamin K3 (menadione) using a flow injection (FI) assembly provided with a solid-phase reactor with immobilized zinc is described. The naphthohydroquinone was produced by means of two coupled steps in the FI system: hydrolysis of the sodium sulfite derivative of the menadione in a basic medium and reduction of the generated menadione in the zinc reactor in an acidic medium. The fluorescent product was monitored spectrofluorimetrically (λex 325 nm; λem 425 nm). The calibration graph was linear over the range 0.1–18 µg ml-1 with a reproducibility of 1.6%; the limit of detection was 0.005 µg ml-1 and the sample throughput was 70 h-1. The influence of foreign compounds was studied and the procedure was applied to the determination of vitamin K3 in three different pharmaceutical formulations.

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