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DOI: 10.1039/A606833B (Paper) Reference Section for: Analyst, 1997, 122, 161-163

Detection of Monensin Residues in Poultry Liver Using an Enzyme Immunoassay

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Steven R. H. Crooks, Imelda M. Traynor, Christopher T. Elliott and William J. McCaughey

Abstract

Monensin, a carboxylic acid ionophore, is commonly fed to poultry to control coccidiosis. A method for the detection and quantification of monensin residues in liver has been developed. Samples (3 g) were extracted with acetonitrile–water and applied to a competitive enzyme immunoassay using a polyclonal antiserum raised against a monensin–transferrin conjugate. The limit of detection (mean + 3s) calculated from the analysis of 12 known negative samples was 2.91 ng g-1. Intra- and inter-assay RSD were determined as 8.5 and 10.6%, respectively, using a liver sample fortified with 20 ng g -1 monensin. A pharmacokinetic study in which 70 six week old broilers were fed monensin at a rate of 120 mg kg-1 in their feed for 14 d resulted in mean monensin liver residues of 102 ng g-1. However these had fallen below the limit of detection of the assay within the 3 d withdrawal period recommended by the manufacturer.

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