High-performance Liquid Chromatographic Determination of Some Amino Acids After Derivatization With 2-Hydroxy-1-naphthaldehyde

Abstract

2-Hydroxy-1-naphthaldehyde (HNA) was investigated as a derivatizing reagent for the fluorescence detection of histidine, methionine and tryptophan at 385 and 265 nm. The separation of HNA-derivatized amino acids on a conventional reversed-phase column was achieved in less than 40 min. This method was compared with the reported o-phthaldialdehyde (OPA) method. The procedure was successfully applied to the assay of the pure amino acids in a mixture and their pharmaceutical dosage forms.

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