DOI: 10.1039/A701036B
(Paper)
Reference Section for: Anal. Commun., 1997, 34, 145-146
Studies on Neurosteroids. Part VI. Characterization of Fatty Acid Esters of Pregnenolone and Dehydroepiandrosterone in Rat Brains Using Liquid Chromatography–Mass Spectrometry
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Kazutake Shimada, Yoshiyuki Mukai, Aki Nakajima and Youko Naka
Abstract
The separation and characterization of 3-fatty acid esters (stearate, palmitate) of pregnenolone and dehydroepiandrosterone in rat brains were carried out using liquid chromatography atmospheric pressure chemical ionization mass spectrometry (LC–APCI-MS) operating in the positive-ion mode. The fatty acid esters obtained from rat brains were derivatized with O-methylhydroxylamine to give the respective methyloximes, which were identified by comparison with authentic samples during the course of LC–APCI-MS.
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