Microwave-induced Plasma Atomic Emission Spectrometer Used as the Detector for On-line Coupled High-performance Liquid Chromatography–Gas Chromatography

Abstract

Atmospheric helium microwave-induced plasma atomic emission spectrometry (MIP-AES) was used successfully as a mode of detection for on-line coupled LC–GC. A loop-type interface was used for the on-line coupling. An early solvent vapor exit was used between the retention gap and the separation capillary column to accelerate solvent evaporation. The interface between MIP-AES and GC was achieved using a TM₀₁₀ cavity operated at atmospheric pressure. On-line coupled LC–GC provided a high efficiency separation for analytes in a complex sample matrix using LC as an on-line clean-up and enrichment approach. MIP-AES offered simultaneous multi-element detection ability which provided valuable information for the correct identification of compounds.

References

1. I. L. Davies, K. E. Markkides, M. L. Lee, M. W. Raynor and K. D. Bartle, J. High Resolut. Chromatogr., 1989, 12, 193.
2. K. Grob, in On-Line Coupled LC–GC, ed. Bertsch, W., Jennings, W. G., and Sandra, P., Hüthig, Heidelberg, 1991, pp. 1–422.
3. L. Mondello and G. Dugo, J. Microcolumn Separations, 1996, 8, 275.
4. T. H. M. Noij and M. M. E. van der Kooi, J. High Resolut. Chromatogr., 1995, 18, 535.
5. T. Hyötyläinen, R. Pilviö and M.-L. Riekkola, J. High Resolut. Chromatogr., 1996, 19, 439.
6. S. Moret, K. Grob and L. S. Conte, J. High Resolut. Chromatogr., 1996, 19, 434.
7. F. Lanuzza, G. Micali and G. Calabrò, J. High Resolut. Chromatogr., 1996, 19, 444.
8. T. V. Raglione, J. A. Troskosky and R. A. Hartwick, J. Chromatogr., 1987, 409, 205.
9. L. Mondello, K. D. Bartle, G. Dugo and P. Dugo, J. High Resolut. Chromatogr., 1994, 17, 312.
10. E. Grimvall, C. Östman and U. Nilsson, J. High Resolut. Chromatogr., 1995, 18, 685.
11. G. Full, G. Krammer and P. Schreier, J. High Resolut. Chromatogr., 1991, 14, 160.
12. A. J. McCormack, S. C. Tong and W. D. Cooke, Anal. Chem., 1965, 37, 147.
13. P. C. Uden, in Elemental Specific Detection by Atomic Emission Spectroscopy ACS Symposium Series, No. 479, ed. Uden, P. C., American Chemical Society, Washington, DC, 1992, pp. 1–24.
14. F. D. Rinkema, A. J. H. Louter and U. A. Th. Brinkman, J. Chromatogr. A, 1994, 678, 289.
15. C.-R. Jia, Y.-R. Huang and L. Jia, Fenxi Yiqi, 1989, 4, 24.
16. K. Grob, Jr and J. M. Stoll, J. High Resolut. Chromatogr. Chromatogr. Commun., 1986, 9, 518.