Demonstration of the difference in binding affinity between the two binding sites of the ristocetin A asymmetric dimer

Abstract

The titration of ¹³C labeled N-acetyl-D-ala-D-ala with the glycopeptide antibiotics ristocetin A and eremomycin, monitored by ¹³C NMR spectroscopy, demonstrates that the two distinct binding sites of the ristocetin A asymmetric dimer have different affinities, whereas those of eremomycin show no detectable difference.