Low absorbance differential spectrophotometry

Abstract

A low absorbance differential spectrophotometry method is proposed. This method is investigated theoretically and experimentally. Its precision and accuracy are compared with those of ordinary spectrophotometry. The results of the proposed method are better than those of normal spectrophotometry in the analysis of the low absorbance samples. In addition, the common problem of the nonlinear relationship between differential absorbance and concentration has been resolved in this method.