Electrochemical immobilization of enzymes. Part VI. Microelectrodes for the detection of L-lactate based on flavocytochrome b2 immobilized in a poly(phenol) film

Abstract

The adsorption of flavocytochrome b₂ at a 25 µm diameter platinum microelectrode, followed by immobilization in an electrochemically polymerized phenol film, was found to be a reproducible method for the fabrication of microelectrodes responsive to L-lactate. Fabrication of enzyme microelectrodes in this manner is simple and produces electrodes that respond rapidly to L-lactate. The immobilization in the poly(phenol) film has no effect on the specificity of the enzyme for L-lactate over β-chlorolactate and reduces the direct electrochemical interference from uric acid, acetomidophenol and ascorbate. The stability of the resulting enzyme electrode is discussed.