A trifunctional compound, incorporating a photolabel, a coenzyme, and an analyte of interest, is synthesized and used to prepare an enzyme reagent that had been modified near its active site.
A. I. MacLean, T. Cynkowskl and L. G. Bachas, J. Chem. Soc., Chem. Commun., 1992, 1283 DOI: 10.1039/C39920001283
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