Biotransformations in carbohydrate synthesis. N-Acetylgalactosaminyl and N-acetylglucosaminyl transfer onto methyl α- and β-glucosides catalysed by the β-N-acetylhexosaminidase from Aspergillus oryzae

Abstract

Regioselectivity of N-acetylhexosaminyl transfer onto methyl α- and β-D-glucosides catalysed by the N-acetyl hexosaminidase from Aspergillus oryzae is strongly dependent on the configuration at the anomeric centre of the acceptor: with the β-glucoside, 1,3- and 1,4-transfer products are formed, whereas with the α-glucoside, 1,4-and 1,6-transfer products are obtained.