Incorporation studies with Aspergillus melleus, using 17O labelled acetate and 17O labelled gas as precursors, and 17O NMR spectroscopy as the analytical tool, have established that all three isomeric metabolites, aspyrone, asperlactone and isoasperlactone, arise from a common diepoxide intermediate.
J. Staunton and A. C. Sutkowski, J. Chem. Soc., Chem. Commun., 1991, 1106 DOI: 10.1039/C39910001106
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