A novel hydrogel matrix on gold surfaces in surface plasmon resonance sensors for fast and efficient covalent immobilization of ligands

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Stefan Löfås and Bo Johnsson


Gold surfaces intended for surface plasmon resonance sensors have been derivatized with a flexible matrix composed of carboxymethylated dextran to provide fast and efficient coupling of proteins and other ligands at low concentrations using the principles of ionic pre-concentration; these hydrophilic and protein compatible hydrogel surfaces allow increased immobilization capacity as compared to monolayer based coatings, for applications in biospecific interaction analysis.


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