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DOI: 10.1039/C39900001526 (Paper) Reference Section for: J. Chem. Soc., Chem. Commun., 1990, 1526-1528

A novel hydrogel matrix on gold surfaces in surface plasmon resonance sensors for fast and efficient covalent immobilization of ligands

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Stefan Löfås and Bo Johnsson

Abstract

Gold surfaces intended for surface plasmon resonance sensors have been derivatized with a flexible matrix composed of carboxymethylated dextran to provide fast and efficient coupling of proteins and other ligands at low concentrations using the principles of ionic pre-concentration; these hydrophilic and protein compatible hydrogel surfaces allow increased immobilization capacity as compared to monolayer based coatings, for applications in biospecific interaction analysis.

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