Acylation monitoring in solid phase peptide synthesis by the equilibrium distribution of coloured ions

Abstract

Spectrophotometric monitoring of the release of anionic dye from the support matrix during the acylation step in solid phase peptide synthesis (counterion distribution monitoring; CDM) is a sensitive indicator of the progress of this often problematic part of the process; this new method is readily incorporated into automated instrumentation where it permits feedback control of the time allowed for the coupling reaction, with consequent improvements in product quality and the speed of synthesis.