The diffusional release of exo-1,4-α-D-glucosidase from agarose gel stops when Concanavalin A binds to the gel, but its removal from the gel by D–mannose leads to rapid release; the reversible initiation–cessation control can be achieved in both batchwise and column operations by the alternate use of D-mannose and Concanavalin A.
E. Kokufuta, I. Nakamura, M. Haraguchi and A. Shimada, J. Chem. Soc., Chem. Commun., 1989, 1564 DOI: 10.1039/C39890001564
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