Use of Fourier transform infrared spectroscopy for quantitative analysis: a comparative study of different detection methods

Abstract

The value of Fourier transform infrared spectroscopy in the mid-infrared region for the quantitative analysis of protein-starch mixtures is examined. Attenuated total reflectance, diffuse reflectance and photoacoustic detection are compared. It is concluded that photoacoustic detection is the best method of analysis for protein but that only attenuated total reflectance is of use in wet systems. Diffuse reflectance gave poorly resolved spectra, which severely limited its usefulness.