Simple and precise high-performance liquid chromatographic method for the investigation of carbodiimide-mediated peptide synthesis

Abstract

A simple HPLC method for the determination of intermediates, products and by-products of peptide synthesis is described. The mean errors of the quantitative determination of all components of the reaction mixture at any stage of peptide synthesis were found to be low (1.3–2.1%). The sensitivity was good enough to detect a 0.05% yield of the products. The method was successfully used to determine the effects of the reaction conditions on optical purity and yield of the synthesised peptide and amino acid derivatives.