Rapid spectrophotometric determination of saccharin in soft drinks and pharmaceuticals using Azure B as reagent

Abstract

Saccharin reacts quantitatively with Azure B in disodium hydrogen orthophosphate - citric acid buffer, forming a blue product that can be extracted into chloroform. Beer's law is valid over the concentration range 2–68 µg ml^–1 of saccharin and the molar absorptivity is 2.4 × 10³ l mol^–1 cm^–1. Reasonable amounts of ingredients that are likely to be present in soft drinks and pharmaceuticals do not interfere. Recoveries of saccharin from soft drinks and pharmaceuticals were satisfactory.