Biosynthesis of aflatoxins. Incorporation of [1,2-13C2]acetate, [2H3]acetate, and [1-13C, 2H3]acetate into sterigmatocystin in Aspergillus versicolor

Abstract

The complete ¹³C–¹³C coupling pattern in sterigmatocystin enriched from [¹³C₂]acetate has been determined and confirms that no fandomisation of labelling in ring-A occurs; ²H-labelling studies indicate that ²H from [²H₃]acetate is retained at C-6 of sterigmatocystin to rule out mechanisms for xanthone ring formation requiring introduction of the phenolic hydroxy-group on this carbon.