Uniformly 13C-enriched substrates as n.m.r probes for metabolic events in vivo. Application of double quantum coherence to a biochemical problem
Abstract
Glycolysis in Escherichia coli has been monitored in vivo by 13C n.m.r. spectroscopy and the biochemical pathways involved in succinate biosynthesis have been evaluated by analysis of the complex resonance signals, using double quantum coherence.