Determination of nicotinamide in some injections of B-complex vitamins by thin-layer chromatography

Abstract

Nicotinamide in some injections of B-complex vitamins can be separated on a thin layer of silica gel G by using a solvent mixture of ethanol (98 per cent.)-chloroform (25 + 60 V/V), eluted from the scraped off area with 0·1 N hydrochloric acid and the solution measured spectrophotometrically at 261 nm. Results obtained by use of the proposed method are compared with those obtained with the ammonia distillation method of the British Pharmacopoeia 1968.